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ATG5 抗体 (N-Term)

Name: ATG5 抗体 (N-Term)
SKU: ABIN1449605
Availability: OutOfStock

ATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

Western blot analysis using ATG5 Antibody (N-term) in Hela cell line lysates (35ug/lane). (ATG5 抗体 (N-Term))

Western blot analysis using ATG5 Antibody (N-term) Cat. (ATG5 抗体 (N-Term))

Formalin-Fixed, Paraffin-Embedded human breast carcinoma tissue reacted with Autophagy ATG5 Antibody (N-term) followed which was peroxidase conjugated to the secondary antibody, followed by DAB staining. (ATG5 抗体 (N-Term))

IHC (p)

Formalin-Fixed, Paraffin-Embedded human cancer tissue reacted with ATG5 Antibody (N-term) followed which was peroxidase conjugated to the secondary antibody, followed by DAB staining. (ATG5 抗体 (N-Term))

IHC (p)

Western blot analysis of APG5L (arrow) using ATG5 Antibody (N-term) Cat. (ATG5 抗体 (N-Term))

Western blot analysis of ATG5 Antibody (N-term) in Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. (ATG5 抗体 (N-Term))

6 images

产品编号 ABIN1449605

发货至: 中国

Datasheet as PDF

抗原 See all ATG5 抗体

ATG5 (ATG5 Autophagy Related 5 (ATG5))
抗原表位
16

11

7

6

6

5

5

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1
AA 1-30, N-Term
适用
108

47

40

6

6

5

5

4

4

4

4

3

3

2
人, 小鼠
宿主
83

22

4

1
兔
Compatible Secondaries
克隆类型
85

25
多克隆
标记
52

8

7

6

6

6

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This ATG5 antibody is un-conjugated
应用范围
73

54

35

17

14

14

13

13

10

7

4

3

3

2

2

2

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

特异性

This antibody recognizes Human and Mouse ATG5 (N-term). Other species not tested.

纯化方法

Saturated Ammonium Sulfate precipitation followed by dialysis against PBS

免疫原

KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of Human APG5L Genename: ATG5

亚型

Ig Fraction

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anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibody
ATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 209-238), (C-Term) antibody
ATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibody
ATG5 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 3C7A8 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibody
ATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibody
ATG5 适用: 人 WB, ELISA, IHC, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibody
ATG5 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) (AA 28-275) antibody
ATG5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG5 Autophagy Related 5 (ATG5) antibody
KO Validated ATG5 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Liquid

浓度

0.25 mg/mL

缓冲液

PBS containing 0.09 % (W/V) Sodium Azide as preservative

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

ATG5 (ATG5 Autophagy Related 5 (ATG5))

别名

APG5L / ATG5 (ATG5 产品)

别名

APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody, ATG5 antibody, CG1643 antibody, DmAtg5 antibody, Dmel\\CG1643 antibody, atg5 antibody, 2010107M05Rik antibody, 3110067M24Rik antibody, AW319544 antibody, Apg5l antibody, Atg5l antibody, C88337 antibody, Paddy antibody, apg5l antibody, zgc:100934 antibody, ATATG5 antibody, AUTOPHAGY 5 antibody, MKP11.20 antibody, MKP11_20 antibody, autophagy related 5 antibody, Autophagy-related 5 antibody, ATG5 autophagy related 5 homolog (S. cerevisiae) antibody, autophagy related 5 L homeolog antibody, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 antibody, autophagy protein Apg5 family antibody, ATG5 antibody, Atg5 antibody, atg5 antibody, atg5.L antibody, APG5 antibody

背景

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5

分子量

32447 Da

基因ID

9474

NCBI登录号

NP_004840

途径

Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy