Ammonium sulfate precipitation and affinity chromatography on protein A agarose
免疫原
Recombinant E. coli RecA protein
克隆位点
ARM193
亚型
IgG2b
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
溶解方式
Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water.
浓度
1.0 mg/mL (following reconstitution)
缓冲液
PBS ( pH 7.2), 0.09 % NaN3, 1 % sucrose
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted in aliquots at -20 °C.
抗原
Recombinase A / RecA
背景
RecA protein (38 kDa) is important in various cellular functions such as DNA repair, genetic recombination, induction of prophages, mutagenesis, cell division, and DNA replication in E. coli. RecA has DNA-dependent ATPase activity, and promotes ATP-dependent pairing of double stranded DNA and homologous single stranded fragments to form D-loops.Synonyms: lexB, recH, rnmB, tif, umuB, zab