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CD8B 抗体 (AA 51-150)

Name: CD8B 抗体 (AA 51-150)
SKU: ABIN1387300
Availability: OutOfStock

CD8B 适用: 人 WB, ELISA, IHC (fro), IF (p), IF (cc), ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-CD8B Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD8B 抗体 (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD8B Polyclonal Antibody, Unconjugated (ABIN1387300) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at (CD8B 抗体 (AA 51-150))

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8B Polyclonal Antibody, Unconjugated (ABIN1387300) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (CD8B 抗体 (AA 51-150))

IHC

3 images

产品编号 ABIN1387300

发货至: 中国

Datasheet as PDF

抗原 See all CD8B 抗体

CD8B (CD8b Molecule (CD8B))
抗原表位
14

11

8

8

7

4

3

3

2

2

2

2

1

1

1

1

1
AA 51-150
适用
66

25

20

14

6

5

4

4

4

4

3

3

2

2

2

1

1

1

1

1
人
宿主
66

47

16
兔
Compatible Secondaries
克隆类型
67

61

1
多克隆
标记
61

16

15

9

6

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD8B antibody is un-conjugated
应用范围
71

69

34

26

16

15

12

12

8

7

3

3

3

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

交叉反应

人

预测反应

Mouse,Rat,Pig,Horse,Chicken,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human CD8B

亚型

IgG
anti-CD8b Molecule (CD8B) antibody
CD8B 适用: 大鼠 FACS, WB, IP, IHC (fro) 宿主: 小鼠 Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (FITC)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 FITC

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal 341 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) (AA 1-243) antibody
CD8B 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-CD8b Molecule (CD8B) antibody
CD8B 适用: 大鼠 FACS, WB, IP, IHC (fro), Func 宿主: 小鼠 Monoclonal 341 unconjugated

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: Cow, 山羊, 绵羊, Water Buffalo FACS 宿主: 小鼠 Monoclonal CC58 PE

anti-CD8b Molecule (CD8B) antibody (PE)
CD8B 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal H35-17-2 PE

anti-CD8b Molecule (CD8B) (AA 22-170) antibody
CD8B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
抗原

CD8B (CD8b Molecule (CD8B))

别名

CD8B (CD8B 产品)

背景

Synonyms: LY3, P37, LEU2, LYT3, CD8B1, T-cell surface glycoprotein CD8 beta chain, CD8b
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

基因ID

926

UniProt

P10966

途径

TCR Signaling