Working Dilutions: Flow Cytometry FITC and BIOT conjugates 2 g/106 cells PE, APC, and SPRD conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
样本量
1 mL
限制
仅限研究用
浓度
0.1 mg/mL
缓冲液
0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/Sodium azide and a stabilizing agent
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
CD24 is expressed in B cells until their terminal differentiation into plasma cells. It has been used widely as a marker to fractionate different B-lineage differentiation stages. CD24 has been found on a variety of other hematopoietic and neural cell types, and is now classified as a costimulatory molecule. The CD24/HSA molecule is composed mostly of carbohydrate, with a protein core of only 31-35 amino acids in the human and 27 AA in the mouse. The molecular mass is variable, ranging from 38-70 kDa. CD24 is now included in the large group of phosphatidylinositol-anchored membrane proteins. Studies with purified CD24 applied to latex beads have demonstrated a self-binding property, suggesting that the molecule is capable of promoting homotypic aggregation, binding and cell adhesion. This is supported by earlier observations of the blockade of B cell lymphoblast aggregation with anti-CD24 monoclonal antibody. Recent studies of murine CD24 have assigned a role for CD24/HSA as a ligand for P-selectin, a calcium-dependent lectin.