CD45.1 抗体 (Biotin)
-
- 抗原 See all CD45.1 products
- CD45.1 (CD45 (CD45.1 Allogen))
- 适用
- 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This CD45.1 antibody is conjugated to Biotin
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- Mouse CD45.1, Mr 180-240 kDa
- 产品特性
- Mouse Anti-Mouse CD45.1-BIOT
- 免疫原
- SJL mouse thymocytes and splenocytes
- 克隆位点
- A20
- 亚型
- IgG2a
-
-
- 应用备注
-
- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PACBLU conjugate 0.3 g/106 cells PE, APC, SPRD, CY5, PE/CY5.5, PE/CY7, APC/CY5.5, 0.2 g/106 cells APC/CY7, and AF647 conjugates For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- 样本量
- 1 mL
- 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- 0.5 mg in 1.0 mL of PBS/Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
-
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C
-
- 抗原
- CD45.1 (CD45 (CD45.1 Allogen))
- 别名
- CD45.1 (CD45.1 产品)
- 背景
- The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation.
-