CD32/CD16 抗体
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- 抗原 See all CD32/CD16 products
- CD32/CD16
- 适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD32/CD16 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 特异性
- Mouse CD16/32
- 产品特性
- Rat Anti-Mouse CD16/32-UNLB
- 纯化方法
- Purified
- 免疫原
- VH81X Tg B cells and T220 stromal cell line
- 克隆位点
- 93
- 亚型
- IgG2a
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- 应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Block - Reported in literature
- Working Dilutions: Flow Cytometry Purified (UNLB) antibody 3 g/106 cells FITC and BIOT conjugates 3 g/106 cells PE, APC, SPRD, PE/CY5.5, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- 说明
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Blocking of g receptors
- 样本量
- 1 mL
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
- 储存液
- Without preservative
- 注意事项
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C
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- 抗原
- CD32/CD16
- 别名
- CD16/32 (CD32/CD16 产品)
- 背景
- The lymphocyte Fc receptors recognize the Fc portion of IgG, presented either as immune complexes or as free antibody. The different classes of receptors are distinct because of varying size, tissue distribution and affinity for IgGs. The Fc type II receptor is expressed on a wide variety of cells including B cells, hematopoietic cells, monocyte/macrophages, neutrophils, platelets, Langerhans cells, eosinophils, basophils, trophoblasts, and endothelial cells of the placenta. The Fc type III receptors are higher affinity than the type II and are expressed on macrophages, NK cells and neutrophils. Both types of receptors can be expressed on the same cell and in varying ratios. The receptors are constitutively expressed, although cytokines and lymphokines can modulate their expression.4 Besides identifying FcR+ cells, monoclonal antibodies to the FcII/III receptor have been used to block Fc receptor binding of IgG, Fc-mediated signal transduction and effector functions, clearance of immune complexes and to attenuate infection by organisms dependent on FcR for parasitic invasion.
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