Applications: FC - Quality tested , IHC-FS - Reported in literature , IP - Reported in literature , Neut - Reported in literature , Block - Reported in literature , Depletion - Reported in literature , CMCD - Reported in literature
Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE, APC, SPRD, and PE/CY7 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
样本量
1 mL
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
0.5 mg of purified immunoglobulin in 1.0 mL of PBS
储存液
Azide free
注意事项
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件
-20 °C
储存方法
Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.
The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus.