(2 references)-
- 抗原 See all LY9 (CD229) 抗体
- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LY9 antibody is conjugated to PE
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应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody HLy9.25 (also known as HLy9.1.25) recognizes an extracellular epitope of CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- 免疫原
- CD299-transfected 300.19 pre-B cell line
- 克隆位点
- HLy9-25
- 亚型
- IgG1
- Top Product
- Discover our top product CD229 Primary Antibody
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anti-Lymphocyte Antigen 9 (CD229) (AA 384-642) antibody
CD229 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 9 (CD229) antibodyCD229 适用: 人 WB, FACS, IP, ICC, Func 宿主: 小鼠 Monoclonal HLy9-25 unconjugated
anti-Lymphocyte Antigen 9 (CD229) (AA 160-360) antibodyCD229 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 9 (CD229) antibodyCD229 适用: 人 WB, FACS, IP, ICC 宿主: 小鼠 Monoclonal HLy9-25 unconjugated
anti-Lymphocyte Antigen 9 (CD229) antibody (APC)CD229 适用: 人 FACS 宿主: 小鼠 Monoclonal HLy9-25 APC
anti-Lymphocyte Antigen 9 (CD229) (AA 2-212) antibodyCD229 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 9 (CD229) (AA 301-400) antibodyCD229 适用: 人 ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lymphocyte Antigen 9 (CD229) (AA 156-253) antibodyCD229 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
- 说明
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- 限制
- 仅限研究用
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- 溶解方式
- No reconstitution is necessary.
- 缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
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Do not freeze.
Avoid prolonged exposure to light. - 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).
: "Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9." in: Blood, Vol. 97, Issue 11, pp. 3513-20, (2001) (PubMed).
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: "CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 11, pp. 7033-42, (2005) (PubMed).
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- 抗原
- LY9 (CD229) (Lymphocyte Antigen 9 (CD229))
- 别名
- CD229 (CD229 产品)
- 别名
- LY9 antibody, CD229 antibody, SLAMF3 antibody, hly9 antibody, mLY9 antibody, AI893573 antibody, Lgp100 antibody, Ly-9 antibody, T100 antibody, lymphocyte antigen 9 antibody, LY9 antibody, Ly9 antibody
- 背景
- Lymphocyte antigen 9,CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.,SLAMF3, LY9
- 基因ID
- 4063
- UniProt
- Q9HBG7
- 途径
- BCR Signaling
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