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Nitrotyrosine 抗体

适用: Various Species WB, IHC (fro), IHC (p) 宿主: 小鼠 Monoclonal EM-30 unconjugated
产品编号 ABIN1302502
发货至: 中国
  • 抗原 See all Nitrotyrosine 抗体
    Nitrotyrosine
    适用
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    Various Species
    宿主
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    小鼠
    克隆类型
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    单克隆
    标记
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    This Nitrotyrosine antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
    交叉反应 (详细)
    Broad
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    免疫原
    NO2-Tyr-CH2-Thyroglobulin
    克隆位点
    EM-30
    亚型
    IgG1
    Top Product
    Discover our top product Nitrotyrosine Primary Antibody
  • 应用备注
    Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
    Western blotting: positive control: NO2-BSA, NO2-HSA.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • 抗原
    Nitrotyrosine
    Abstract
    Nitrotyrosine 产品
    物质类
    Chemical
    背景
    Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
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