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- 抗原 See all LIME (LIME1) 抗体
- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
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抗原表位
- AA 86-202
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This LIME antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP)
- 特异性
- Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
- 交叉反应 （详细）
- Mouse, Other not tested
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Recombinant protein corresponding to amino acids 86-202 of mouse LIME
- 克隆位点
- MLIME-05
- 亚型
- IgG2a
- Top Product
- Discover our top product LIME1 Primary Antibody
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anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody
LIME1 适用: 人 IP, FACS 宿主: 小鼠 Monoclonal LIME-06 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 170-295) antibodyLIME1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 281-296) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal LIME-10 unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibodyLIME1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 141-295) antibody (PE)LIME1 适用: 人 FACS 宿主: 小鼠 Monoclonal LIME-06 PE
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (C-Term) antibodyVerified LIME1 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Lck Interacting Transmembrane Adaptor 1 (LIME1) (AA 1-295) antibodyLIME1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))
- 别名
- LIME (LIME1 产品)
- 别名
- LIME1 antibody, DKFZp459A158 antibody, LIME antibody, LP8067 antibody, dJ583P15.4 antibody, 2810038K19Rik antibody, RGD1562795 antibody, Lck interacting transmembrane adaptor 1 antibody, LIME1 antibody, Lime1 antibody
- 背景
- Lck interacting transmembrane adaptor 1,LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.,Lita1
- 基因ID
- 72699
- UniProt
- Q9EQR5
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