我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MHC Class II 抗体

Name: MHC Class II 抗体
Brand: antibodies-online
SKU: ABIN1301917
Availability: OutOfStock

MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p), Func 宿主: 大鼠 Monoclonal M5-114 unconjugated

Flow cytometry analysis (surface staining) of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC. (MHC Class II 抗体)

FACS

1 image

(8 references)

产品编号 ABIN1301917

发货至: 中国

Datasheet as PDF

抗原 See all MHC Class II (MHC2) 抗体

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
适用
48

45

13

9

9

6

4

2

2

1

1
小鼠
宿主
45

27

23
大鼠
Compatible Secondaries
克隆类型
75

19

1
单克隆
标记
28

15

10

10

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MHC Class II antibody is un-conjugated
应用范围
67

39

21

20

17

13

13

8

5

3

2

2

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

特异性

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

交叉反应（详细）

Mouse

纯化方法

Purified by protein-G affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Activated C57BL/6 mouse spleen cells

克隆位点

M5-114

亚型

IgG2b

Top Product

Discover our top product MHC2 Primary Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)
MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (SPRD)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 SPRD

应用备注

Functional application: Blocking of T cell proliferative responses.
Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Kuwano, Prazma, Yazawa, Watanabe, Ishiura, Kumanogoh, Okochi, Tamaki, Fujimoto, Tedder: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).

Beers, Burich, Kleijmeer, Griffith, Wong, Rudensky: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).

Zang, Kalache, Lin, Schroppel, Murphy: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).

Kleijmeer, Ramm, Schuurhuis, Griffith, Rescigno, Ricciardi-Castagnoli, Rudensky, Ossendorp, Melief, Stoorvogel, Geuze: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." in: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).

Clausen, Waldburger, Schwenk, Barras, Mach, Rajewsky, Förster, Reith: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." in: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).

De Souza Leao, Lang, Prina, Hellio, Antoine: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." in: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).

Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
抗原

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

别名

MHC Class II (MHC2 产品)

别名

H-2 antibody, MHC-II antibody, histocompatibility-2, MHC antibody, H2 antibody

背景

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.