我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

MHC Class II 抗体

Name: MHC Class II 抗体
Brand: antibodies-online
SKU: ABIN1301917
Availability: OutOfStock

MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p), Func 宿主: 大鼠 Monoclonal M5-114 unconjugated

Flow cytometry analysis (surface staining) of MHCII on murine splenocytes with anti-MHCII (M5/114) azide free, GAR-APC. (MHC Class II 抗体)

FACS

1 image

(8 references)

产品编号 ABIN1301917

发货至: 中国

Datasheet as PDF

抗原 See all MHC Class II (MHC2) 抗体

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
适用
48

45

14

9

9

6

4

2

2

1

1
小鼠
宿主
45

28

23
大鼠
Compatible Secondaries
克隆类型
76

19

1
单克隆
标记
29

15

10

10

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This MHC Class II antibody is un-conjugated
应用范围
68

39

21

21

18

13

13

8

5

3

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func)

特异性

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

交叉反应（详细）

Mouse

纯化方法

Purified by protein-G affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Activated C57BL/6 mouse spleen cells

克隆位点

M5-114

亚型

IgG2b

Top Product

Discover our top product MHC2 Primary Antibody
anti-Major Histocompatibility Complex Class II Antigen (MHC2) (AA 111-200) antibody
MHC2 适用: 小鼠, 人 WB, IHC (fro), IHC (p), IF (cc), IF (p), ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal M5-114 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (Biotin)
MHC2 适用: 小鼠 FACS, WB, IHC (fro), IP, IHC (p) 宿主: 大鼠 Monoclonal M5-114 Biotin

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal 2G11 unconjugated

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody
MHC2 适用: 小鼠, 大鼠 FACS, IHC, IP 宿主: 兔 Chimeric P7-7 unconjugated Recombinant Antibody

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (PE)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 PE

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (APC)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 APC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (FITC)
MHC2 适用: 犬 FACS 宿主: 大鼠 Monoclonal YKIX334-2 FITC

anti-Major Histocompatibility Complex Class II Antigen (MHC2) antibody (SPRD)
MHC2 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal NIMR-4 SPRD

应用备注

Functional application: Blocking of T cell proliferative responses.
Flow cytometry: Recommended dilution: 2 μg/mL.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4

储存液

Azide free

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Kuwano, Prazma, Yazawa, Watanabe, Ishiura, Kumanogoh, Okochi, Tamaki, Fujimoto, Tedder: "CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells." in: International immunology, Vol. 19, Issue 8, pp. 977-92, (2007) (PubMed).

Beers, Burich, Kleijmeer, Griffith, Wong, Rudensky: "Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 3, pp. 1205-12, (2005) (PubMed).

Zang, Kalache, Lin, Schroppel, Murphy: "MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 12, pp. 3661-8, (2005) (PubMed).

Kleijmeer, Ramm, Schuurhuis, Griffith, Rescigno, Ricciardi-Castagnoli, Rudensky, Ossendorp, Melief, Stoorvogel, Geuze: "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells." in: The Journal of cell biology, Vol. 155, Issue 1, pp. 53-63, (2001) (PubMed).

Clausen, Waldburger, Schwenk, Barras, Mach, Rajewsky, Förster, Reith: "Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice." in: Immunity, Vol. 8, Issue 2, pp. 143-55, (1998) (PubMed).

De Souza Leao, Lang, Prina, Hellio, Antoine: "Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells." in: Journal of cell science, Vol. 108 ( Pt 10), pp. 3219-31, (1995) (PubMed).

Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).
抗原

MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

别名

MHC Class II (MHC2 产品)

背景

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.