Vesicular Stomatitis Virus G Protein (VSV G) (C-Term) 抗体 (Biotin)
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- 抗原
- Vesicular Stomatitis Virus G Protein (VSV G)
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抗原表位
- C-Term
- 适用
- 请咨询
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Supplier Product No.
- 600-406-386
- Supplier
- Rockland
- 原理
- VSV-G Epitope Tag Antibody Biotin Conjugated
- 序列
- YTDIEMNRLG K
- 交叉反应 （详细）
- This affinity purified antibody is directed against VSV-G protein.
- 产品特性
- Synonyms: Rabbit Anti-VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti VSV-G Epitope Tag Biotin Conjugated Antibody, Rabbit Anti-VSV-G Tag Antibody Biotin Conjugation
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification followed by biotin conjugation, subsequent purification and exhaustive dialysis against the buffer stated above.
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
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anti-Vesicular Stomatitis Virus G Protein (VSV G) (AA 501-511) antibody
VSV G ELISA, FM, IHC, WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: This affinity purified antibody has been tested for use in ELISA and western blot and is suitable by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluorescence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:50,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Vesicular Stomatitis Virus G Protein (VSV G)
- 别名
- VSV-G
- 物质类
- Viral Protein
- 背景
- Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pH CMV-VSV-G.
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