VSV-g Tag 抗体 (Biotin)
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- 抗原 See all VSV-g Tag products
- VSV-g Tag (VSV-G Epitope Tag)
- 适用
- Vesicular Stomatitis Virus (VSV)
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VSV-g Tag antibody is conjugated to Biotin
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应用范围
- ELISA, Fluorescence Microscopy (FM)
- 序列
- YTDIEMNRLG K
- 产品特性
- Concentration Definition: by UV absorbance at 280 nm
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G).
- 亚型
- IgG
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anti-VSV-G Epitope Tag antibody
适用: Vesicular Stomatitis Virus (VSV) WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) WB, IF, IP 宿主: 小鼠 Monoclonal 8D6 unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibodyWB, IF, IP 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibody适用: Vesicular Stomatitis Virus (VSV) WB, IF, IP, IC 宿主: 小鼠 Monoclonal unconjugated
anti-VSV-G Epitope Tag (AA 501-511) antibody适用: Vesicular Stomatitis Virus (VSV) WB 宿主: 山羊 Polyclonal unconjugated
anti-VSV-G Epitope Tag antibodyWB 宿主: 兔 Polyclonal RB0418 unconjugated
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- 应用备注
- This affinity purified antibody has been tested for use in ELISA and by immunofluorescence microscopy. Specific conditions for reactivity should be optimized by the end user. For standard indirect immunofluor-escence assays, we recommend paraformaldehyde fixation with detergent permeabilization of cells infected with VSV or transfected with the wild-type VSV-G protein. Staining is quite clean and specific for both sources of the G protein when diluted at least 1/200. At higher concentrations, a filamentous background staining may be present.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
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- 抗原
- VSV-g Tag (VSV-G Epitope Tag)
- 别名
- VSV-G Tag (VSV-g Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. VSV-G, or vesicular stomatitis virus glycoprotein, is found within the pseudo lentiviral cloning vector pHCMV-VSV-G.
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