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RFP 抗体 (HRP)

Rockland 600-403-379 RFP 适用: Discosoma WB, ELISA, IHC 宿主: 兔 Polyclonal HRP
Rockland
产品编号 ABIN129725
Supplier Product No.: 600-403-379
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    适用
    Discosoma
    宿主
    • 21
    • 13
    • 9
    • 5
    • 3
    克隆类型
    • 30
    • 21
    多克隆
    标记
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    This RFP antibody is conjugated to HRP
    应用范围
    • 25
    • 19
    • 14
    • 11
    • 11
    • 8
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-403-379
    Supplier
    Rockland
    原理
    RFP Antibody Peroxidase Conjugated Pre-Adsorbed
    交叉反应 (详细)
    Minimal crossreactivity against Hu Ms & Rt Serum Proteins
    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
    产品特性
    Synonyms: rabbit anti-RFP antibody peroxidase conjugation, rabbit anti-Red Fluorescent Protein antibody peroxidase conjugation, HRP conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    纯化方法

    Rabbit Anti-RFP Peroxidase Conjugated Antibody was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Preadsorption: Pre-adsorbed

    免疫原

    Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

    Immunogen Type: Recombinant Protein

    亚型
    IgG
    Top Product
    Discover our top product RFP Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: Rabbit Anti-RFP Peroxidase Conjugated Antibody has been tested in ELISA and Western blot. It can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen or to detect RFP or RFP containing proteins on Western blots. References suggest that the antibody is also suitable for IHC. Optimal titers for applications should be determined by the researcher.

    Western Blot Dilution: 1:2,000 - 1:5,000

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    别名
    DsRed (RFP 产品)
    背景
    Background: Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19 % identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.
    UniProt
    Q9U6Y8
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