GPX4 抗体 (C-Term)
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- 抗原 See all GPX4 抗体
- GPX4 (Glutathione Peroxidase 4 (GPX4))
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抗原表位
- C-Term
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适用
- 小鼠, 大鼠, 豚鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPX4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Supplier Product No.
- 600-401-972
- Supplier
- Rockland
- 原理
- Glutathione Peroxidase 4 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against mouse GPx4 protein.
- 产品特性
- Synonyms: rabbit anti-Gpx4 antibody, rabbit anti-glutathione peroxidase 4/Gpx4 antibody, rabbit anti-glutathione peroxidase 4 antibody, PHGPx antibody, Phospholipid hydroperoxide glutathione peroxidase antibody, Gpx-4, Gpx 4
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse GPx4 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product GPX4 Primary Antibody
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anti-Glutathione Peroxidase 4 (GPX4) (AA 30-197) antibody
GPX4 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (AA 74-197) antibodyGPX4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (AA 74-197) antibodyGPX4 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (C-Term) antibodyGPX4 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (C-Term) antibodyVerified GPX4 适用: 人 WB, IHC, ELISA, IP 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) antibodyKO Validated GPX4 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (AA 74-197) antibodyGPX4 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) antibodyGPX4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 4 (GPX4) antibodyGPX4 适用: 人, 小鼠, 大鼠 IHC, ELISA, IF, FACS, Coat, StM 宿主: 小鼠 Monoclonal LHM 2 unconjugated
anti-Glutathione Peroxidase 4 (GPX4) (AA 72-121) antibodyGPX4 适用: 人, 小鼠, 大鼠, 斑马鱼, 豚鼠, Cow, 猴, 山羊 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 19 kDa in size corresponding to GPx4 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:7,000 - 1:30,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.15 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- GPX4 (Glutathione Peroxidase 4 (GPX4))
- 别名
- Gpx4 (GPX4 产品)
- 背景
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Glutathione Peroxidase 4 (GPx4, also known as PHGPx) functions as an antioxidant and also plays a role in redox regulation, sexual maturation, inflammation, apoptosis, and differentiation. GPx4 was found to have an important role in preventing lipid peroxidation, and has been discussed primarily as the glutathione peroxidase protecting biomembranes against oxidative stress. GPx4 can react with free hydrogen peroxide as well as with a wide range of lipid hydroperoxides, including those derived from cholesterol and cholesteryl esters. GPx4 is the only GPx to use phospholipid hydroperoxides as substrates. It can also reduce thymine hydroperoxide. In contrast to GPx1 and GPx3, GPx4 can use a wide range of reducing substrates in addition to glutathione. GPx4 is expressed in high levels in testes, suggesting a role in male fertility. It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm.
- 基因ID
- 625249, 13508533
- UniProt
- O70325
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