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PDCD4 抗体 (C-Term)

Name: PDCD4 抗体 (C-Term)
SKU: ABIN129709
Availability: OutOfStock

Rockland 600-401-965 PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

H2O2 causes cytoplasmic accumulation of HuR and a loss in PDCD4 expression that is mediated by miR-21A. (PDCD4 抗体 (C-Term))

Western blot using affinity purified anti-Pdcd4 antibody shows detection of a band ~52 kDa in size corresponding to Pdcd4 (arrowhead). (PDCD4 抗体 (C-Term))

Western Blot of Rabbit anti-PDCD antibody. (PDCD4 抗体 (C-Term))

HuR regulates PDCD4 stability via miR-21A. (PDCD4 抗体 (C-Term))

HuR directly binds to PDCD4 3'UTR mRNA to regulate its protein expressionA. (PDCD4 抗体 (C-Term))

ERK8 phosphorylates HuR to prevent its binding to PDCD4 mRNAA. (PDCD4 抗体 (C-Term))

8 images

(11 references)

产品编号 ABIN129709

Supplier Product No.: 600-401-965

发货至: 中国

Datasheet as PDF

抗原 See all PDCD4 抗体

PDCD4 (Programmed Cell Death 4 (PDCD4))
抗原表位
15

8

8

7

6

5

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
C-Term
适用
96

62

51

6

5

4

4

3

3

3

3

3

3

1

1
人, 小鼠
宿主
89

5

2

1
兔
Compatible Secondaries
克隆类型
85

12
多克隆
标记
58

4

3

3

3

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This PDCD4 antibody is un-conjugated
应用范围
73

41

29

14

14

14

12

9

6

3

3

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-401-965

Supplier

Rockland

原理

Pdcd4 Antibody

交叉反应（详细）

This affinity purified antibody is directed against human Pdcd4 protein.

产品特性

Synonyms: rabbit anti-PDCD4 antibody, PDCD-4, PDCD 4, Programmed cell death protein 4, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody, Protein 197/15a

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.
Immunogen Type: Conjugated Peptide

亚型

IgG
anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-100) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-469) antibody
PDCD4 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (N-Term) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer67) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) antibody
PDCD4 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 121-149) antibody
PDCD4 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB33836 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (pSer457) antibody
PDCD4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 9G6 unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-260) antibody
PDCD4 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

anti-Programmed Cell Death 4 (PDCD4) (AA 1-260) antibody
PDCD4 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:100 - 1:300
Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, immunoprecipitation and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:10,000
ELISA Dilution: 1:10,000 - 1:100,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.06 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Hill, Nesser, Johnson-Camacho, Jeffress, Johnson, Boniface, Spencer, Lu, Heiser, Lawrence, Pande, Korkola, Gray, Mills, Mukherjee, Spellman: "Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling." in: Cell systems, Vol. 4, Issue 1, pp. 73-83.e10, (2019) (PubMed).

Kim, Hu, Jadhav, Jin, Zhang, Cavanagh, Akondy, Ahmed, Weyand, Goronzy: "Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells." in: Cell reports, Vol. 25, Issue 8, pp. 2148-2162.e5, (2018) (PubMed).

Liwak-Muir, Dobson, Naing, Wylie, Chehade, Baird, Chakraborty, Holcik: "ERK8 is a novel HuR kinase that regulates tumour suppressor PDCD4 through a miR-21 dependent mechanism." in: Oncotarget, Vol. 7, Issue 2, pp. 1439-50, (2016) (PubMed).

Sharma, Lin, Farrugia, McLaughlin, Ellis, Brundage, Salkeni, Ruppert: "MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1." in: Molecular and cellular biology, Vol. 34, Issue 22, pp. 4143-64, (2015) (PubMed).

Yang, Yue, Sims, Pfeffer: "The curcumin analog EF24 targets NF-κB and miRNA-21, and has potent anticancer activity in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 8, pp. e71130, (2014) (PubMed).

Hirata, Suzuki, Imaeda, Matsuzaki, Tsugawa, Nagano, Asakura, Saya, Hibi: "CD44 variant 9 expression in primary early gastric cancer as a predictive marker for recurrence." in: British journal of cancer, Vol. 109, Issue 2, pp. 379-86, (2013) (PubMed).

Ruan, Wang, Kameswaran, Wei, Johnson, Matschinsky, Shi, Chen: "The microRNA-21-PDCD4 axis prevents type 1 diabetes by blocking pancreatic beta cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 29, pp. 12030-5, (2011) (PubMed).

Santhanam, Baker, Hegamyer, Kirschmann, Colburn: "Pdcd4 repression of lysyl oxidase inhibits hypoxia-induced breast cancer cell invasion." in: Oncogene, Vol. 29, Issue 27, pp. 3921-32, (2010) (PubMed).

OHayre, Salanga, Kipps, Messmer, Dorrestein, Handel: "Elucidating the CXCL12/CXCR4 Signaling Network in Chronic Lymphocytic Leukemia through Phosphoproteomics Analysis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11716, (2010) (PubMed).

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).
抗原

PDCD4 (Programmed Cell Death 4 (PDCD4))

别名

PDCD4 (PDCD4 产品)

背景

Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

基因ID

27250, 21735596

UniProt

Q53EL6