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ECT2 抗体 (pThr790)

Name: ECT2 抗体 (pThr790)
SKU: ABIN129688
Availability: OutOfStock

Rockland 600-401-935 ECT2 适用: 人 WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated

Western blot using affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. (ECT2 抗体 (pThr790))

1 image

产品编号 ABIN129688

Supplier Product No.: 600-401-935

发货至: 中国

Datasheet as PDF

抗原 See all ECT2 抗体

ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))
抗原表位
15

7

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
pThr790
适用
56

12

4

3

3

3

2

2

2

1

1
人
宿主
51

4

1
兔
Compatible Secondaries
克隆类型
52

4
多克隆
标记
22

7

5

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This ECT2 antibody is un-conjugated
应用范围
41

18

14

14

14

8

4

2

2

1

1

1

1
Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.

600-401-935

Supplier

Rockland

原理

ECT2 phospho T790 Antibody

交叉反应（详细）

This affinity-purified antibody is directed against the phosphorylated form of human ECT2 protein at the pT790 residue.

产品特性

Synonyms: rabbit anti-ECT2 pT790 Antibody, ECT 2 antibody, ECT-2 protein antibody, Epithelial cell transforming sequence 2 antibody

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human ECT2 protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

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应用备注

Application Note: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract. Less than 2 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue. Experiments to generate more conclusive evidence are currently under preparation.
Western Blot Dilution: 1:1,000 - 1:4,000
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 1:10,000 - 1:40,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.1 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
抗原

ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

别名

ECT2 (ECT2 产品)

背景

Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42. ECT2 may be phosphorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division. Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.

基因ID

1894, 21735572

UniProt

Q9H8V3

途径

Neurotrophin Signaling Pathway, Cell-Cell Junction Organization