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EIF3F 抗体 (AA 100-125)

Rockland 600-401-934 EIF3F 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129687
Supplier Product No.: 600-401-934
发货至: 中国
  • 抗原 See all EIF3F 抗体
    EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
    抗原表位
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 100-125
    适用
    • 30
    • 20
    • 10
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 27
    • 3
    克隆类型
    • 30
    多克隆
    标记
    • 23
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EIF3F antibody is un-conjugated
    应用范围
    • 22
    • 11
    • 8
    • 6
    • 6
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    600-401-934
    Supplier
    Rockland
    原理
    EIF3F Antibody
    交叉反应 (详细)
    This affinity-purified antibody is directed against human eIF3f protein.
    产品特性
    Synonyms: rabbit anti-eIF3F Antibody, eIF-3 epsilon antibody, eIF3 p47 antibody, eIF3-p47 subunit antibody, EIF3S5 antibody, Eukaryotic translation initiation factor 3 subunit 5 antibody, Eukaryotic translation initiation factor 3 subunit F, Deubiquitinating enzyme eIF3f
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human eIF3f protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product EIF3F Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:200 - 1:2,000

    ELISA Dilution: 1:10,000 - 1:40,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • You, Dooley, Kim, Kim, Xu, Goodman, Hornberger: "A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling." in: Science signaling, Vol. 11, Issue 530, (2018) (PubMed).

    Ham, Caldow, Chhen, Chee, Wang, Proud, Lynch, Koopman: "Glycine restores the anabolic response to leucine in a mouse model of acute inflammation." in: American journal of physiology. Endocrinology and metabolism, Vol. 310, Issue 11, pp. E970-81, (2017) (PubMed).

    Wada, Kato, Okutsu, Miyaki, Suzuki, Yan, Schiaffino, Asahara, Ushida, Akimoto: "Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy." in: The Journal of biological chemistry, (2011) (PubMed).

  • 抗原
    EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))
    别名
    EIF3F (EIF3F 产品)
    背景
    Background: eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.
    基因ID
    8665, 6685511
    UniProt
    O00303
    途径
    Ribonucleoprotein Complex Subunit Organization
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