This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 50 kDa in size corresponding to phosphorylated Mer2 protein by western blotting in the appropriate cell lysate or extract. Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for Mer2 phosphorylated at the pS30 residue. Preparation of extracts from cells 4hr after initiation of meiosis is suggested.
限制
仅限研究用
状态
Liquid
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that S-phase CDK associates with the replication fork. Synonyms: Meiotic recombination 2 protein REC107