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MARK2 抗体 (pThr595)

Rockland 600-401-895 MARK2 适用: 人 WB, ELISA, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129661
Supplier Product No.: 600-401-895
发货至: 中国
  • 抗原 See all MARK2 抗体
    MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))
    抗原表位
    • 13
    • 8
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    pThr595
    适用
    • 75
    • 53
    • 44
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    宿主
    • 71
    • 3
    • 1
    克隆类型
    • 64
    • 11
    多克隆
    标记
    • 35
    • 4
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    • 3
    • 3
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    • 3
    • 1
    • 1
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    This MARK2 antibody is un-conjugated
    应用范围
    • 44
    • 31
    • 16
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    • 11
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    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-895
    Supplier
    Rockland
    原理
    MARK2 phospho T595 Antibody
    交叉反应 (详细)
    MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.
    产品特性
    Synonyms: rabbit anti-MARK2 pT595 antibody, MARK-2, MARK 2, ELKL motif kinase 1, EMK1, EMK-1, MAP/microtubule affinity regulating kinase 2 antibody, PAR1 homolog antibody, Serine/threonine protein kinase MARK2 antibody, Par-1b, Par1b, PAR1 homolog b
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a.  The immunogen peptide was phosphorylated at the residue corresponding to T595.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product MARK2 Primary Antibody
  • 应用备注

    Application Note: Anti-MARK2pT595 antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:250 - 1:1,000

    ELISA Dilution: 1:1,000 - 1:3,000

    IF Microscopy Dilution: User Optimized

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.6 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))
    别名
    MARK2 (MARK2 产品)
    背景
    Background: MARK2 pT595 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses.  MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens].  EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer.  Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2.  These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it.  Both forms were found to be co-expressed in a number of selected cell lines and tissue samples.  Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed.  This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein.   Multiple splice variants exist for this protein.
    基因ID
    2011
    UniProt
    Q7KZI7
    途径
    SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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