MARK2 抗体 (pThr595)
-
- 抗原 See all MARK2 抗体
- MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))
-
抗原表位
- pThr595
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This MARK2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-895
- Supplier
- Rockland
- 原理
- MARK2 phospho T595 Antibody
- 交叉反应 (详细)
- MARK2 pT595 affinity purified antibody is directed against the phosphorylated form of human MARK2 at the pT595 residue.
- 产品特性
- Synonyms: rabbit anti-MARK2 pT595 antibody, MARK-2, MARK 2, ELKL motif kinase 1, EMK1, EMK-1, MAP/microtubule affinity regulating kinase 2 antibody, PAR1 homolog antibody, Serine/threonine protein kinase MARK2 antibody, Par-1b, Par1b, PAR1 homolog b
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- 过滤
- Sterile filtered
- 免疫原
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding T595 of Human MARK2 isoform a. The immunogen peptide was phosphorylated at the residue corresponding to T595.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product MARK2 Primary Antibody
-
-
- 应用备注
-
Application Note: Anti-MARK2pT595 antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 83 kDa corresponding to MARK2 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:250 - 1:1,000
ELISA Dilution: 1:1,000 - 1:3,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.6 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- MARK2 (MAP/microtubule Affinity-Regulating Kinase 2 (MARK2))
- 别名
- MARK2 (MARK2 产品)
- 背景
- Background: MARK2 pT595 antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. This antibody recognizes a regulatory phosphorylation on a kinase that is critical to regulation of microtubules, cell cycle and immune responses. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encode two isoforms of the human ser/thr protein kinase EMK1 called MARK2. These isoforms were characterized by the presence of a 162-bp alternative exon that gives rise to two forms, one containing the exon and the other one lacking it. Both forms were found to be co-expressed in a number of selected cell lines and tissue samples. Human MARK2 was shown to be encoded by a single mRNA that is ubiquitously expressed. This transcription variant includes the alternative exon in the coding region and therefore codes for a longer protein. Multiple splice variants exist for this protein.
- 基因ID
- 2011
- UniProt
- Q7KZI7
- 途径
- SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
-