我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

beta Actin 抗体 (AA 359-368)

Name: beta Actin 抗体 (AA 359-368)
Brand: antibodies-online
SKU: ABIN129657
Availability: OutOfStock

ACTB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit Anti-Beta Actin Antibody. (beta Actin 抗体 (AA 359-368))

Immunohistochemistry of Rabbit Anti-Beta Actin Antibody. (beta Actin 抗体 (AA 359-368))

Distinct functions of HOXD proteins in regulation of neuronal differentiation. (beta Actin 抗体 (AA 359-368))

E2F6 is essential for HOXC9 induction of G1 arrest and repression of cell cycle genes. (beta Actin 抗体 (AA 359-368))

Effects of TRPM2 inhibition and RNAi knockdown in cancerous and non-cancerous breast cell lines after chemotherapeutic treatments. (beta Actin 抗体 (AA 359-368))

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments. (beta Actin 抗体 (AA 359-368))

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing. (beta Actin 抗体 (AA 359-368))

10 images

(31 references)

产品编号 ABIN129657

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
40

18

16

6

6

5

4

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 359-368
适用
192

138

120

65

52

44

37

37

32

25

21

20

12

10

9

8

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
人
宿主
114

94

5

1

1
兔
Compatible Secondaries
克隆类型
113

100
多克隆
标记
129

14

13

11

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
175

77

59

51

35

30

27

22

13

13

6

6

6

5

5

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

交叉反应

大鼠, 人, 猴, 绵羊, 牛, 山羊, 豚鼠, 猪, Hamster

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

亚型

IgG

Top Product

Discover our top product ACTB Primary Antibody
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人 WB, IF, IP 宿主: 小鼠 Monoclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), FACS, DNA Mic 宿主: 小鼠 Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

应用备注

Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Olive, Qin, DeCristo, Laszewski, Greathouse, McAllister: "Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies." in: PLoS ONE, Vol. 13, Issue 6, pp. e0198790, (2019) (PubMed).

Dai, Luftig: "Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 5, pp. 1727-1736, (2019) (PubMed).

Borroni, Emanuelli, Shah, Ilić, Apel-Sarid, Paolini, Manikoth Ayyathan, Koganti, Levy-Cohen, Blank: "Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin." in: Aging cell, Vol. 17, Issue 2, (2018) (PubMed).

Coleman, Ha, Haeryfar, Barr, Kim: "Cathepsin B plays a key role in optimal production of the influenza A virus." in: Journal of virology & antiviral research, Vol. 2018, pp. 1-20, (2018) (PubMed).

Lodola, Laforenza, Cattaneo, Ruffinatti, Poletto, Massa, Tancredi, Zuccolo, Khdar, Riccardi, Biggiogera, Rosti, Guerra, Moccia: "VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells." in: Oncotarget, Vol. 8, Issue 56, pp. 95223-95246, (2017) (PubMed).

Chen, Wang, Man, Li, Shan: "Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice." in: Oncology letters, Vol. 13, Issue 4, pp. 2207-2215, (2017) (PubMed).

Rae, Hocum, Bii, Deeg, Trobridge: "A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis." in: Oncotarget, Vol. 6, Issue 31, pp. 30664-74, (2016) (PubMed).

Koh, Powell, Blake, Hoffman, Hopkins, Feng: "Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel." in: Oncology reports, Vol. 34, Issue 3, pp. 1589-98, (2016) (PubMed).

Campos, Qiu, Gomero, Wakefield, Horner, Brutkowski, Han, Solecki, Frase, Bongiovanni, dAzzo: "Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier." in: Nature communications, Vol. 7, pp. 11876, (2016) (PubMed).

Hopkins, Feng, Liu, Parker, Koh: "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." in: International journal of oncology, Vol. 46, Issue 5, pp. 2267-76, (2015) (PubMed).

Maragkoudaki, Vaiopoulou, Theodoropoulos, Legaki, Sechi, Karamanolis, Zografos, Gazouli: "Specific detection of OCT4 isoforms in inflammatory bowel disease." in: Gut pathogens, Vol. 7, pp. 25, (2015) (PubMed).

Zha, Xia, Ding, Choi, Yang, Dong, Yan, Huang, Ding: "MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression." in: Cell death & disease, Vol. 5, pp. e1417, (2015) (PubMed).

Nelson, Estus, Abner, Parikh, Malik, Neltner, Ighodaro, Wang, Wilfred, Wang, Kukull, Nandakumar, Farman, Poon, Corrada, Kawas, Cribbs, Bennett, Schneider, Larson, Crane, Valladares, Schmitt, Kryscio et al.: "ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. ..." in: Acta neuropathologica, Vol. 127, Issue 6, pp. 825-43, (2015) (PubMed).

Wang, Choi, Ding, Yang, Ngoka, Lee, Zha, Mao, Jin, Ren, Cowell, Huang, Shi, Cui, Ding: "HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation." in: BMC genomics, Vol. 14, pp. 830, (2014) (PubMed).

Dragoni, Laforenza, Bonetti, Reforgiato, Poletto, Lodola, Bottino, Guido, Rappa, Pareek, Tomasello, Guarrera, Cinelli, Aronica, Guerra, Barosi, Tanzi, Rosti, Moccia: "Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis." in: PLoS ONE, Vol. 9, Issue 3, pp. e91099, (2014) (PubMed).

Dragoni, Turin, Laforenza, Potenza, Bottino, Glasnov, Prestia, Ferulli, Saitta, Mosca, Guerra, Rosti, Luinetti, Ganini, Porta, Pedrazzoli, Tanzi, Montagna, Moccia: "Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma." in: BioMed research international, Vol. 2014, pp. 739494, (2014) (PubMed).

Wang, Danaher, Miller, Berger, Nubia, Wilfred, Neltner, Norris, Nelson: "Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells." in: Genomics, proteomics & bioinformatics, Vol. 12, Issue 1, pp. 19-30, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." in: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Laforenza, Scaffino, Gastaldi: "Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes." in: PLoS ONE, Vol. 8, Issue 1, pp. e54474, (2013) (PubMed).

Lodola, Laforenza, Bonetti, Lim, Dragoni, Bottino, Ong, Guerra, Ganini, Massa, Manzoni, Ambudkar, Genazzani, Rosti, Pedrazzoli, Tanzi, Moccia, Porta: "Store-operated Ca2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients." in: PLoS ONE, Vol. 7, Issue 9, pp. e42541, (2013) (PubMed).

show more references
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

beta Actin (ACTB 产品)

别名

BRWS1 antibody, PS1TP5BP1 antibody, ACTA1 antibody, Actx antibody, E430023M04Rik antibody, beta-actin antibody, ps1tp5bp1 antibody, act-2 antibody, actb antibody, B-actin antibody, LOC100174883 antibody, LOC100304816 antibody, A antibody, ACT5C antibody, Ac5C antibody, Act antibody, Act-5C antibody, Act5 antibody, Act5c antibody, Actin antibody, Actin/BAP47 antibody, Actin5C antibody, BAP47 antibody, Bap47 antibody, CG4027 antibody, Dmel\\CG4027 antibody, M32055 antibody, T11 antibody, act antibody, act 5C antibody, act5C antibody, actin antibody, actin5C antibody, anon-EST:fe2D2 antibody, beta-actin/Bap47 antibody, cyt5C antibody, l(1)G0009 antibody, l(1)G0010 antibody, l(1)G0025 antibody, l(1)G0079 antibody, l(1)G0117 antibody, l(1)G0177 antibody, l(1)G0245 antibody, l(1)G0330 antibody, l(1)G0420 antibody, l(1)G0486 antibody, Bact antibody, ACT-5 antibody, ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactin1 antibody, bactzf antibody, wu:fd18f05 antibody, A26C1B antibody, POTE2alpha antibody, POTEACTIN antibody, Beta-actin antibody, actin beta antibody, actin, beta antibody, cytoplasmic actin OlCA1 antibody, beta-actin antibody, Actin 5C antibody, actin gamma 1 antibody, actin, beta 1 antibody, actin, beta L homeolog antibody, beta actin antibody, POTE ankyrin domain family member F antibody, actin, beta like 2 antibody, actin, cytoplasmic 1 antibody, ACTB antibody, Actb antibody, actb antibody, LOC100174883 antibody, LOC100304816 antibody, Act5C antibody, ACTG1 antibody, actb1 antibody, actb.L antibody, LOC100136352 antibody, POTEF antibody, ACTBL2 antibody, LOC109073280 antibody

背景

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
Synonyms: Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

基因ID

4501885

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction