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beta Actin 抗体 (AA 350-375)

Name: beta Actin 抗体 (AA 350-375)
SKU: ABIN129657
Availability: OutOfStock

Rockland 600-401-886 ACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated

Western Blot of Rabbit Anti-Beta Actin Antibody. (beta Actin 抗体 (AA 350-375))

Immunohistochemistry of Rabbit Anti-Beta Actin Antibody. (beta Actin 抗体 (AA 350-375))

Distinct functions of HOXD proteins in regulation of neuronal differentiation. (beta Actin 抗体 (AA 350-375))

E2F6 is essential for HOXC9 induction of G1 arrest and repression of cell cycle genes. (beta Actin 抗体 (AA 350-375))

Effects of TRPM2 inhibition and RNAi knockdown in cancerous and non-cancerous breast cell lines after chemotherapeutic treatments. (beta Actin 抗体 (AA 350-375))

Analysis of poly(ADP-ribose)-mediated caspase-independent cell death in breast adenocarcinoma cells after TRPM2 inhibition and chemotherapeutic treatments. (beta Actin 抗体 (AA 350-375))

TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing. (beta Actin 抗体 (AA 350-375))

10 images

(31 references)

产品编号 ABIN129657

Supplier Product No.: 600-401-886

发货至: 中国

Datasheet as PDF

抗原 See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))
抗原表位
41

16

15

6

6

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 350-375
适用
181

130

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
人, 小鼠, Leopard Frog
宿主
115

82

5

1

1
兔
Compatible Secondaries
克隆类型
112

90
多克隆
标记
129

13

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
应用范围
171

73

54

46

33

31

26

22

13

13

8

6

6

6

5

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.

600-401-886

Supplier

Rockland

原理

Beta Actin Antibody

交叉反应（详细）

beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse.

产品特性

Synonyms: rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

过滤

Sterile filtered

免疫原

Immunogen: beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near amino acids 350-375 of Human beta Actin.
Immunogen Type: Conjugated Peptide

亚型

IgG

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应用备注

Application Note: Anti-beta Actin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000 - 1:4,000
ELISA Dilution: 1:10,000 - 1:40,000
IF Microscopy Dilution: 1:500 - 1:2,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Olive, Qin, DeCristo, Laszewski, Greathouse, McAllister: "Accounting for tumor heterogeneity when using CRISPR-Cas9 for cancer progression and drug sensitivity studies." in: PLoS ONE, Vol. 13, Issue 6, pp. e0198790, (2019) (PubMed).

Dai, Luftig: "Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 200, Issue 5, pp. 1727-1736, (2019) (PubMed).

Borroni, Emanuelli, Shah, Ilić, Apel-Sarid, Paolini, Manikoth Ayyathan, Koganti, Levy-Cohen, Blank: "Smurf2 regulates stability and the autophagic-lysosomal turnover of lamin A and its disease-associated form progerin." in: Aging cell, Vol. 17, Issue 2, (2018) (PubMed).

Coleman, Ha, Haeryfar, Barr, Kim: "Cathepsin B plays a key role in optimal production of the influenza A virus." in: Journal of virology & antiviral research, Vol. 2018, pp. 1-20, (2018) (PubMed).

Lodola, Laforenza, Cattaneo, Ruffinatti, Poletto, Massa, Tancredi, Zuccolo, Khdar, Riccardi, Biggiogera, Rosti, Guerra, Moccia: "VEGF-induced intracellular Ca2+ oscillations are down-regulated and do not stimulate angiogenesis in breast cancer-derived endothelial colony forming cells." in: Oncotarget, Vol. 8, Issue 56, pp. 95223-95246, (2017) (PubMed).

Chen, Wang, Man, Li, Shan: "Increased B7-H4 expression during esophageal squamous cell carcinogenesis is associated with IL-6/STAT3 signaling pathway activation in mice." in: Oncology letters, Vol. 13, Issue 4, pp. 2207-2215, (2017) (PubMed).

Rae, Hocum, Bii, Deeg, Trobridge: "A novel retroviral mutagenesis screen identifies prognostic genes in RUNX1 mediated myeloid leukemogenesis." in: Oncotarget, Vol. 6, Issue 31, pp. 30664-74, (2016) (PubMed).

Koh, Powell, Blake, Hoffman, Hopkins, Feng: "Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel." in: Oncology reports, Vol. 34, Issue 3, pp. 1589-98, (2016) (PubMed).

Campos, Qiu, Gomero, Wakefield, Horner, Brutkowski, Han, Solecki, Frase, Bongiovanni, dAzzo: "Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier." in: Nature communications, Vol. 7, pp. 11876, (2016) (PubMed).

Hopkins, Feng, Liu, Parker, Koh: "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." in: International journal of oncology, Vol. 46, Issue 5, pp. 2267-76, (2015) (PubMed).

Maragkoudaki, Vaiopoulou, Theodoropoulos, Legaki, Sechi, Karamanolis, Zografos, Gazouli: "Specific detection of OCT4 isoforms in inflammatory bowel disease." in: Gut pathogens, Vol. 7, pp. 25, (2015) (PubMed).

Zha, Xia, Ding, Choi, Yang, Dong, Yan, Huang, Ding: "MEIS2 is essential for neuroblastoma cell survival and proliferation by transcriptional control of M-phase progression." in: Cell death & disease, Vol. 5, pp. e1417, (2015) (PubMed).

Nelson, Estus, Abner, Parikh, Malik, Neltner, Ighodaro, Wang, Wilfred, Wang, Kukull, Nandakumar, Farman, Poon, Corrada, Kawas, Cribbs, Bennett, Schneider, Larson, Crane, Valladares, Schmitt, Kryscio et al.: "ABCC9 gene polymorphism is associated with hippocampal sclerosis of aging pathology. ..." in: Acta neuropathologica, Vol. 127, Issue 6, pp. 825-43, (2015) (PubMed).

Wang, Choi, Ding, Yang, Ngoka, Lee, Zha, Mao, Jin, Ren, Cowell, Huang, Shi, Cui, Ding: "HOXC9 directly regulates distinct sets of genes to coordinate diverse cellular processes during neuronal differentiation." in: BMC genomics, Vol. 14, pp. 830, (2014) (PubMed).

Dragoni, Laforenza, Bonetti, Reforgiato, Poletto, Lodola, Bottino, Guido, Rappa, Pareek, Tomasello, Guarrera, Cinelli, Aronica, Guerra, Barosi, Tanzi, Rosti, Moccia: "Enhanced expression of Stim, Orai, and TRPC transcripts and proteins in endothelial progenitor cells isolated from patients with primary myelofibrosis." in: PLoS ONE, Vol. 9, Issue 3, pp. e91099, (2014) (PubMed).

Dragoni, Turin, Laforenza, Potenza, Bottino, Glasnov, Prestia, Ferulli, Saitta, Mosca, Guerra, Rosti, Luinetti, Ganini, Porta, Pedrazzoli, Tanzi, Montagna, Moccia: "Store-operated Ca2+ entry does not control proliferation in primary cultures of human metastatic renal cellular carcinoma." in: BioMed research international, Vol. 2014, pp. 739494, (2014) (PubMed).

Wang, Danaher, Miller, Berger, Nubia, Wilfred, Neltner, Norris, Nelson: "Expression of miR-15/107 family microRNAs in human tissues and cultured rat brain cells." in: Genomics, proteomics & bioinformatics, Vol. 12, Issue 1, pp. 19-30, (2014) (PubMed).

Papaconstantinou, Zeglinas, Gazouli, Nastos, Yiallourou, Lykoudis, Evangelou, Papalois, Papaioannou, Vlachogiannakos, Tzathas: "Effect of infliximab on the healing of intestinal anastomosis. An experimental study in rats." in: International journal of surgery (London, England), Vol. 12, Issue 9, pp. 969-75, (2014) (PubMed).

Laforenza, Scaffino, Gastaldi: "Aquaporin-10 represents an alternative pathway for glycerol efflux from human adipocytes." in: PLoS ONE, Vol. 8, Issue 1, pp. e54474, (2013) (PubMed).

Lodola, Laforenza, Bonetti, Lim, Dragoni, Bottino, Ong, Guerra, Ganini, Massa, Manzoni, Ambudkar, Genazzani, Rosti, Pedrazzoli, Tanzi, Moccia, Porta: "Store-operated Ca2+ entry is remodelled and controls in vitro angiogenesis in endothelial progenitor cells isolated from tumoral patients." in: PLoS ONE, Vol. 7, Issue 9, pp. e42541, (2013) (PubMed).

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抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB (ACTB 产品)

背景

Background: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

NCBI登录号

NP_001092

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction