CDC27 抗体
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- 抗原 See all CDC27 抗体
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDC27 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)
- Supplier Product No.
- 600-401-885
- Supplier
- Rockland
- 原理
- CDC27 phospho T244 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against the phosphorylated form of human CDC27 at the pT244 residue.
- 产品特性
- Synonyms: rabbit anti-CDC27 pT244 Antibody, CDC 27, CDC-27, Cell division cycle protein 27 homolog, H-NUC, APC3, Anaphase-promoting complex subunit 3, ANAPC3
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion surrounding T244 of Human CDC27.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product CDC27 Primary Antibody
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anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 551-830) antibody
CDC27 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibodyCDC27 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 5C12 unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 258-431) antibodyCDC27 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 1-830) antibodyCDC27 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 395-444) antibodyCDC27 适用: 人, 大鼠, Cow, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Middle Region) antibodyCDC27 适用: 人, 大鼠, 犬, Cow, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (Center) antibodyCDC27 适用: 人, Cow WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 814-823), (C-Term) antibodyCDC27 适用: 人, 大鼠, 小鼠, 犬, Cow, 非洲爪蟾 WB, IP, IHC (p), EIA 宿主: 小鼠 Monoclonal AF3-1 unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 92 kDa in size corresponding to CDC27 by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pT244 of CDC27.
Western Blot Dilution: 1:300 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:8,000 - 1:30,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))
- 别名
- CDC27, D0S1430E (CDC27 产品)
- 背景
- Background: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
- 基因ID
- 996, 167466175
- UniProt
- P30260
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