TUBA1B 抗体 (AA 425-451)
(18 references)
Quick Overview for TUBA1B 抗体 (AA 425-451) (ABIN1043885)
抗原
See all TUBA1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 425-451
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Supplier Product No.
- 600-401-880s
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Supplier
- Rockland
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原理
- Alpha-Tubulin Antibody
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交叉反应 （详细）
- Anti-Tubulin Loading Control Antibody is directed against human alpha Tubulin protein.
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产品特性
- Synonyms: rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
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纯化方法
- The Loading Control Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Tubulin, alpha 1B (TUBA1B) antibody
TUBA1B 适用: 人 WB, IF, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D1 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF, FM, MA 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 425-451) antibodyTUBA1B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, FM 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 29-310) antibodyTUBA1B 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2E8 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (C-Term) antibodyTUBA1B 适用: 人, 小鼠, 大鼠, 小鸡, Cow WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 17H11-F10 unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 437-441) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tubulin, alpha 1B (TUBA1B) (AA 1-451) antibodyTUBA1B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-Tubulin Antibody has been tested for use in ELISA, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in most cell lysates or extracts.
Western Blot Dilution: 1:1,000 - 1:5,000
ELISA Dilution: 1:90,000
IF Microscopy Dilution: 1:500 - 1:2,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.1 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-Tubulin Loading Control Antibody at -20° C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3." in: Scientific reports, Vol. 8, Issue 1, pp. 8680, (2018) (PubMed).
: "FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1." in: Oncotarget, Vol. 9, Issue 45, pp. 27708-27727, (2018) (PubMed).
: "The RNA exosome contributes to gene expression regulation during stem cell differentiation." in: Nucleic acids research, Vol. 46, Issue 21, pp. 11502-11513, (2018) (PubMed).
: "Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression." in: Scientific reports, Vol. 7, pp. 46239, (2018) (PubMed).
: "Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling." in: Acta neuropathologica communications, Vol. 5, Issue 1, pp. 70, (2018) (PubMed).
: "Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations." in: The Journal of comparative neurology, Vol. 524, Issue 14, pp. 2845-72, (2017) (PubMed).
: "The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module." in: Nucleic acids research, Vol. 45, Issue 20, pp. 11989-12004, (2017) (PubMed).
: "Relationships between PROMPT and gene expression." in: RNA biology, Vol. 13, Issue 1, pp. 6-14, (2016) (PubMed).
: "Nicotinamide ameliorates palmitate-induced ER stress in hepatocytes via cAMP/PKA/CREB pathway-dependent Sirt1 upregulation." in: Biochimica et biophysica acta, Vol. 1853, Issue 11 Pt A, pp. 2929-36, (2015) (PubMed).
: "Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates." in: The EMBO journal, Vol. 33, Issue 10, pp. 1159-76, (2014) (PubMed).
: "The prolyl isomerase, FKBP25, interacts with RNA-engaged nucleolin and the pre-60S ribosomal subunit." in: RNA (New York, N.Y.), Vol. 20, Issue 7, pp. 1014-22, (2014) (PubMed).
: "Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells." in: Journal of molecular signaling, Vol. 8, Issue 1, pp. 9, (2013) (PubMed).
: "FBXW7α attenuates inflammatory signalling by downregulating C/EBPδ and its target gene Tlr4." in: Nature communications, Vol. 4, pp. 1662, (2013) (PubMed).
: "Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity." in: Nature communications, Vol. 4, pp. 1801, (2013) (PubMed).
: "Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag." in: Journal of virology, Vol. 86, Issue 6, pp. 2990-3002, (2012) (PubMed).
: "Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells." in: Molecular and cellular biology, Vol. 32, Issue 2, pp. 320-32, (2012) (PubMed).
: "Structural features and restricted expression of a human alpha-tubulin gene." in: Nucleic acids research, Vol. 13, Issue 1, pp. 207-23, (1985) (PubMed).
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: "A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2." in: Cell reports, Vol. 29, Issue 7, pp. 1800-1811.e6, (2019) (PubMed).
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- TUBA1B (Tubulin, alpha 1B (TUBA1B))
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别名
- TUBA1B
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背景
- Background: Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.
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基因ID
- 17986283
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UniProt
- P68363
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途径
- Microtubule Dynamics, M Phase
抗原
-
