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APC1 抗体 (pSer355)

Rockland 600-401-856 ANAPC1 适用: 人 ELISA, WB, IP 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129636
Supplier Product No.: 600-401-856
发货至: 中国
  • 抗原 See all APC1 (ANAPC1) 抗体
    APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))
    抗原表位
    • 26
    • 16
    • 15
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer355
    适用
    • 49
    • 40
    • 24
    宿主
    • 78
    • 2
    克隆类型
    • 78
    • 2
    多克隆
    标记
    • 30
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This APC1 antibody is un-conjugated
    应用范围
    • 40
    • 40
    • 29
    • 14
    • 13
    • 12
    • 10
    • 10
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    Supplier Product No.
    600-401-856
    Supplier
    Rockland
    原理
    APC1 phospho S355 Antibody
    交叉反应 (详细)
    This antibody is specific for phosphorylated human APC1 protein at the pS355 residue.
    产品特性
    Synonyms: rabbit anti-APC1 pS355 Antibody, Anaphase-promoting complex subunit 1, APC-1, APC 1, Cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator
    纯化方法
    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product ANAPC1 Primary Antibody
  • 应用备注

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 215 kDa in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:200 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:10,000 - 1:35,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))
    别名
    ANAPC1, TSG24 (ANAPC1 产品)
    背景
    Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
    基因ID
    64682, 12056971
    UniProt
    Q9H1A4
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