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SLIT2 抗体 (AA 475-500)

Rockland 600-401-846 SLIT2 适用: 人, 小鸡 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129628
Supplier Product No.: 600-401-846
发货至: 中国
  • 抗原 See all SLIT2 抗体
    SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
    抗原表位
    • 15
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 475-500
    适用
    • 24
    • 22
    • 17
    人, 小鸡
    宿主
    • 46
    • 3
    • 2
    • 1
    克隆类型
    • 49
    • 3
    多克隆
    标记
    • 21
    • 10
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SLIT2 antibody is un-conjugated
    应用范围
    • 43
    • 22
    • 13
    • 13
    • 12
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    600-401-846
    Supplier
    Rockland
    原理
    SLIT2 Antibody
    交叉反应 (详细)
    This affinity purified antibody is directed against human SLIT-2 protein.
    产品特性
    Synonyms: rabbit anti-SLIT-2 antibody, SLIT2, SLIT 2, Slit homolog 2 protein, SLIL3, SLIL-3 antibody
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 475-500 of Human SLIT-2 protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product SLIT2 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 2 μg/mL

    Application Note: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~ 165 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  For western blotting block the membrane with goat serum.  Do not block with non-fat dry milk.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:5,000 - 1:50,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    SLIT2 (Slit Homolog 2 (Drosophila) (SLIT2))
    别名
    SLIT2 (SLIT2 产品)
    背景
    Background: SLIT-2 is thought to act as a molecular guidance cue in cellular migration, and its function appears to be mediated by interaction with roundabout homologue receptors.  SLIT-1 and SLIT-2 seem to be essential for midline guidance in the forebrain by acting as repulsive signals preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development SLIT-2 may play a role in guiding commissural axons once they reach the floor plate by modulating the response to netrin.  In vitro, SLIT-2 silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex.  In vitro, only commissural axons that crossed the midline responded to SLIT-2.  In the developing visual system this protein functions as a repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm.  SLIT-2 is a secreted protein predominantly expressed in fetal lung and kidney, and adult spinal cord.  Weak expression is observed in adult adrenal gland, thyroid, trachea and other tissues examined.  Multiple isoforms have been reported for this product.
    基因ID
    9353, 4759146
    UniProt
    O94813
    途径
    Regulation of Actin Filament Polymerization, Regulation of Cell Size, Smooth Muscle Cell Migration
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