GLI2 抗体 (Internal Region)
(5 references)-
- 抗原 See all GLI2 抗体
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
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抗原表位
- Internal Region
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适用
- 人, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLI2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- This affinity-purified antibody is directed against human Gli-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Gli-2 from human and chimpanzee based on the immunizing sequence.
- 产品特性
- Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.
- 过滤
- Sterile filtered
- 免疫原
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This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human Gli-2 (isoform a).
Immunogen Type: Peptide - 亚型
- IgG
- Top Product
- Discover our top product GLI2 Primary Antibody
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anti-GLI Family Zinc Finger 2 (GLI2) (Middle Region) antibody
GLI2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1287-1321), (C-Term) antibodyGLI2 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB49147 unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (Internal Region) antibodyGLI2 适用: 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (C-Term) antibodyGLI2 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 600-649) antibodyGLI2 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 2-20) antibodyGLI2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (N-Term) antibodyGLI2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) (AA 1444-1463), (C-Term) antibodyGLI2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 2 (GLI2) antibodyGLI2 适用: 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot. Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively). Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
- 说明
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Gene Name: GLI2
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- 有效期
- 12 months
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: "Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients." in: The American journal of pathology, Vol. 188, Issue 3, pp. 795-804, (2019) (PubMed).
: "The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 51, pp. 84286-84298, (2018) (PubMed).
: "Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer." in: BMC cancer, Vol. 17, Issue 1, pp. 634, (2018) (PubMed).
: "The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).
: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, (2005) (PubMed).
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: "Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients." in: The American journal of pathology, Vol. 188, Issue 3, pp. 795-804, (2019) (PubMed).
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- 抗原
- GLI2 (GLI Family Zinc Finger 2 (GLI2))
- 别名
- Gli-2 (GLI2 产品)
- 别名
- HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody
- 背景
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Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.
Synonyms: Gli-2, Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein - 基因ID
- 2736
- NCBI登录号
- NP_005261
- UniProt
- P10070
- 途径
- Hedgehog Signaling, Dopaminergic Neurogenesis
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