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GLI2 抗体 (AA 30-65)

Rockland 600-401-845 GLI2 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129627
Supplier Product No.: 600-401-845
发货至: 中国
  • 抗原 See all GLI2 抗体
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    抗原表位
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 30-65
    适用
    • 25
    • 12
    • 4
    • 1
    人, 大鼠
    宿主
    • 25
    • 2
    克隆类型
    • 25
    • 2
    多克隆
    标记
    • 17
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GLI2 antibody is un-conjugated
    应用范围
    • 15
    • 8
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-401-845
    Supplier
    Rockland
    原理
    GLI2 Antibody
    交叉反应 (详细)
    This affinity-purified antibody is directed against human Gli-2 protein.
    产品特性
    Synonyms: rabbit anti-GLI-2 antibody, GLI2, zinc finger protein GLI2, GLI family zinc finger protein 2, Tax helper protein antibody, THP antibody
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-65 of human Gli-2 (isoform a).

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product GLI2 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500 - 1:2,000

    Application Note: This antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. See figure legend for expectations by western blot.  Multiple splice variants have been reported for this protein a, b, g and d (133.3, 131.6, 88.1 and 86.4 kDa respectively).  Detection of Gli-2 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:10,000 - 1:30,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Gonnissen, Isebaert, Perneel, McKee, Van Utterbeeck, Lerut, Verrill, Bryant, Joniau, Muschel, Haustermans: "Patched 1 Expression Correlates with Biochemical Relapse in High-Risk Prostate Cancer Patients." in: The American journal of pathology, Vol. 188, Issue 3, pp. 795-804, (2019) (PubMed).

    Gonnissen, Isebaert, McKee, Dok, Haustermans, Muschel: "The hedgehog inhibitor GANT61 sensitizes prostate cancer cells to ionizing radiation both in vitro and in vivo." in: Oncotarget, Vol. 7, Issue 51, pp. 84286-84298, (2018) (PubMed).

    Gonnissen, Isebaert, Perneel, McKee, Verrill, Bryant, Van Utterbeeck, Lerut, Haustermans, Muschel: "Tissue microarray analysis indicates hedgehog signaling as a potential prognostic factor in intermediate-risk prostate cancer." in: BMC cancer, Vol. 17, Issue 1, pp. 634, (2018) (PubMed).

    Gonnissen, Isebaert, McKee, Muschel, Haustermans: "The Effect of Metformin and GANT61 Combinations on the Radiosensitivity of Prostate Cancer Cells." in: International journal of molecular sciences, Vol. 18, Issue 2, (2017) (PubMed).

    Roessler, Ermilov, Grange, Wang, Grachtchouk, Dlugosz, Muenke: "A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2." in: Human molecular genetics, Vol. 14, Issue 15, pp. 2181-8, (2005) (PubMed).

  • 抗原
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    别名
    GLI2 (GLI2 产品)
    背景
    Background: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila.
    基因ID
    2736
    NCBI登录号
    NP_005261
    UniProt
    P10070
    途径
    Hedgehog Signaling, Dopaminergic Neurogenesis
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