GLI3 抗体 (AA 30-60)
(1 reference)
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- 抗原 See all GLI3 抗体
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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抗原表位
- AA 30-60
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GLI3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)
- Supplier Product No.
- 600-401-694
- Supplier
- Rockland
- 原理
- GLI3 Antibody
- 交叉反应 （详细）
- This affinity-purified antibody is directed against human Gli-3 protein.
- 产品特性
- Synonyms: Rabbit anti-GLI-3 antibody, Transcriptional activator GLI3, Gli 3, GLI3 form of 190 kDa, GLI3 form of 83 kDa
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product GLI3 Primary Antibody
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anti-GLI Family Zinc Finger 3 (GLI3) antibody
GLI3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 800-900) antibodyGLI3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 40-57), (N-Term) antibodyGLI3 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 11-60) antibodyGLI3 适用: 人, 小鼠, 大鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (N-Term) antibodyGLI3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 0.5 mg/mL - 5 μg/mL
Application Note: This antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:6,000 - 1:30,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).
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: "Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence." in: Neurourology and urodynamics, Vol. 37, Issue 8, pp. 2551-2559, (2019) (PubMed).
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- 抗原
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- 别名
- GLI3 (GLI3 产品)
- 背景
- Background: Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.
- 基因ID
- 2737, 119393899
- UniProt
- P10071
- 途径
- Hedgehog Signaling
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