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EGR1 抗体 (AA 85-115)

Name: EGR1 抗体 (AA 85-115)
SKU: ABIN129622
Availability: OutOfStock

Rockland 600-401-693 EGR1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot using Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kDa correspond-ing to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). (EGR1 抗体 (AA 85-115))

Affinity Purified anti-EGR-1 antibody was used at a 10 ug/ml to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. (EGR1 抗体 (AA 85-115))

IHC

2 images

(2 references)

产品编号 ABIN129622

Supplier Product No.: 600-401-693

发货至: 中国

Datasheet as PDF

抗原 See all EGR1 抗体

EGR1 (Early Growth Response 1 (EGR1))
抗原表位
16

9

7

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 85-115
适用
58

37

25

7

6

6

5

5

4

4

1

1

1
人, 小鼠
宿主
50

11

1
兔
Compatible Secondaries
克隆类型
49

13
多克隆
标记
37

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This EGR1 antibody is un-conjugated
应用范围
50

22

17

13

13

10

7

7

6

5

5

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-401-693

Supplier

Rockland

原理

EGR-1 Antibody

交叉反应（详细）

This affinity purified antibody is directed against human EGR-1 protein.

产品特性

Synonyms: rabbit anti-EGR-1 Antibody, EGR1, EGR 1, AT225 antibody, Early growth response 1 antibody, KROX24 antibody, Nerve growth factor-induced protein A antibody, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, ZNF225, Zinc finger protein 225, Zinc finger protein Krox-24

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 85-115 of Human EGR-1.
Immunogen Type: Conjugated Peptide

亚型

IgG

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应用备注

Immunohistochemistry Dilution: 2 μg/mL to 20 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~58 kDa in size corresponding to EGR-1 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:4,000 - 1:16,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

0.93 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Weaver, Hellstrom, Brown, Andrews, Dymov, Diorio, Zhang, Szyf, Meaney: "The methylated-DNA binding protein MBD2 enhances NGFI-A (egr-1)-mediated transcriptional activation of the glucocorticoid receptor." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 369, Issue 1652, (2015) (PubMed).

Hellstrom, Dhir, Diorio, Meaney: "Maternal licking regulates hippocampal glucocorticoid receptor transcription through a thyroid hormone-serotonin-NGFI-A signalling cascade." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 367, Issue 1601, pp. 2495-510, (2012) (PubMed).
抗原

EGR1 (Early Growth Response 1 (EGR1))

别名

EGR1 (EGR1 产品)

背景

Background: EGR-1 (also called Early Growth Response protein 1, Krox-24 protein, ZIF268, Nerve growth factor-induced protein A or NGFI-A, Transcription factor ETR103, and Zinc finger protein 225 or AT225) is a transcriptional regulator that recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site). EGR-1 activates the transcription of target genes whose products are required for mitogenesis and differentiation. EGR-1 is a nuclear protein induced by growth factors. Expression has been identified in a variety of cancers.

基因ID

1958

NCBI登录号

NP_001955

UniProt

P18146

途径

Regulation of Carbohydrate Metabolic Process, Regulation of long-term Neuronal Synaptic Plasticity