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SETDB1 抗体 (AA 1050-1075)

Rockland 600-401-689 SETDB1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129618
Supplier Product No.: 600-401-689
发货至: 中国
  • 抗原 See all SETDB1 抗体
    SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
    抗原表位
    • 15
    • 8
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    AA 1050-1075
    适用
    • 29
    • 18
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    宿主
    • 37
    • 7
    克隆类型
    • 38
    • 6
    多克隆
    标记
    • 25
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SETDB1 antibody is un-conjugated
    应用范围
    • 43
    • 24
    • 13
    • 13
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Supplier Product No.
    600-401-689
    Supplier
    Rockland
    原理
    ESET Antibody
    交叉反应 (详细)
    Reactivity occurs against human ESET protein.
    产品特性
    Synonyms: rabbit anti-ESET antibody, ERG-associated protein with SET domain, rabbit anti-SETDB1 antibody, SETDB-1, SETDB 1, Histone lysine N methyltransferase antibody, Histone-lysine N-methyltransferase SETDB1 antibody, KMT1E antibody, Lysine N-methyltransferase 1E antibody, SET domain bifurcated 1, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4
    纯化方法
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1050-1075 of human ESET.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product SETDB1 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blotting. Western blotting shows reactivity specific for human ESET detecting a band at approximately 170 kDa.  Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane.  Reactivity in other immunoassays is unknown.

    Western Blot Dilution: 1:1,000 - 1:2,500

    ELISA Dilution: 1:10,000 - 1:50,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.17 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Sarraf, Stancheva: "Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly." in: Molecular cell, Vol. 15, Issue 4, pp. 595-605, (2004) (PubMed).

    Wang, An, Cao, Xia, Erdjument-Bromage, Chatton, Tempst, Roeder, Zhang: "mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression." in: Molecular cell, Vol. 12, Issue 2, pp. 475-87, (2003) (PubMed).

    Schultz, Ayyanathan, Negorev, Maul, Rauscher: "SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins." in: Genes & development, Vol. 16, Issue 8, pp. 919-32, (2002) (PubMed).

    Harte, Wu, Carrasquillo, Matera: "Assignment of a novel bifurcated SET domain gene, SETDB1, to human chromosome band 1q21 by in situ hybridization and radiation hybrids." in: Cytogenetics and cell genetics, Vol. 84, Issue 1-2, pp. 83-6, (1999) (PubMed).

  • 抗原
    SETDB1 (SET Domain, Bifurcated 1 (SETDB1))
    别名
    SETDB1 (SETDB1 产品)
    背景
    Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3.  H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.
    基因ID
    9869, 224177469
    UniProt
    Q15047
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