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Aurora Kinase B 抗体 (Internal Region)

Name: Aurora Kinase B 抗体 (Internal Region)
SKU: ABIN129612
Availability: OutOfStock

Rockland 600-401-677 AURKB 适用: 人, 猴 WB, ELISA, IHC, FM 宿主: 兔 Polyclonal unconjugated

Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). (Aurora Kinase B 抗体 (Internal Region))

Bufalin prevents Aurora A recruitment to mitotic centrosomes and Aurora B recruitment to unattached kinetochores(A) HeLa cells were synchronized by a single thymidine treatment, released in the presence or absence of bufalin (100 nM) for 9 h, and stained for phospho-Aurora A (Green), α-tubulin (Red) and DNA (Blue). (Aurora Kinase B 抗体 (Internal Region))

IF (cc)

Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. (Aurora Kinase B 抗体 (Internal Region))

IHC

High Aurora-B activity promotes KT attachment to MT-walls. (Aurora Kinase B 抗体 (Internal Region))

IF (cc)

4 images

(18 references)

产品编号 ABIN129612

Supplier Product No.: 600-401-677

发货至: 中国

Datasheet as PDF

抗原 See all Aurora Kinase B (AURKB) 抗体

Aurora Kinase B (AURKB)
抗原表位
20

19

13

11

10

8

8

7

6

5

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Internal Region
适用
116

65

33

13

4

4

2

1

1

1

1

1
人, 猴
宿主
112

23

3

1
兔
Compatible Secondaries
克隆类型
100

40
多克隆
标记
79

10

7

6

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Aurora Kinase B antibody is un-conjugated
应用范围
83

58

42

34

16

14

14

13

8

6

4

3

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Supplier Product No.

600-401-677

Supplier

Rockland

原理

AURORA KINASE B phospho T232 Antibody

交叉反应（详细）

Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue.

产品特性

Synonyms: rabbit anti-Aurora B pT232 Antibody, Phospho Aurora B, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

纯化方法

The product was affinity purified from monospecific antiserum by immunoaffinity purification.

过滤

Sterile filtered

免疫原

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

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anti-Aurora Kinase B (AURKB) (pThr232) antibody
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anti-Aurora Kinase B (AURKB) (AA 1-255) antibody
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anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
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anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB 适用: 人, 小鼠, 大鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 WB 宿主: 小鼠 Monoclonal AURKB-1521 unconjugated

应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: Phospho pT232 Aurora B antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. See below for specific protocol. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.
Western Blot Dilution: 1:250 - 1:2,000
ELISA Dilution: 1:10,000 - 1:30,000
IF Microscopy Dilution: User Optimized
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.21 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store Phospho Specific Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Barroso-Vilares, Macedo, Reis, Warren, Compton, Logarinho: "Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence." in: EMBO reports, Vol. 21, Issue 5, pp. e49248, (2020) (PubMed).

Achuthankutty, Thakur, Haahr, Hoffmann, Drainas, Bizard, Weischenfeldt, Hickson, Mailand: "Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability." in: The Journal of cell biology, Vol. 218, Issue 12, pp. 3943-3953, (2020) (PubMed).

Novais-Cruz, Alba Abad, van IJcken, Galjart, Jeyaprakash, Maiato, Ferrás: "Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription." in: eLife, Vol. 7, (2019) (PubMed).

Liu, Kwon, Mannino, Yang, Renda, Khodjakov, Pellman: "Nuclear envelope assembly defects link mitotic errors to chromothripsis." in: Nature, Vol. 561, Issue 7724, pp. 551-555, (2019) (PubMed).

Xie, Lin, Wu, Tan, Cheng, Zhang: "Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway." in: Oncotarget, Vol. 9, Issue 17, pp. 13783-13795, (2018) (PubMed).

Skowyra, Allan, Saurin, Clarke: "USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability." in: Cell reports, Vol. 23, Issue 3, pp. 852-865, (2018) (PubMed).

Drpic, Almeida, Aguiar, Renda, Damas, Lewin, Larkin, Khodjakov, Maiato: "Chromosome Segregation Is Biased by Kinetochore Size." in: Current biology : CB, Vol. 28, Issue 9, pp. 1344-1356.e5, (2018) (PubMed).

de Cárcer, Venkateswaran, Salgueiro, El Bakkali, Somogyi, Rowald, Montañés, Sanclemente, Escobar, de Martino, McGranahan, Malumbres, Sotillo: "Plk1 overexpression induces chromosomal instability and suppresses tumor development." in: Nature communications, Vol. 9, Issue 1, pp. 3012, (2018) (PubMed).

Shrestha, Conti, Tamura, Braun, Ramalingam, Cieslinski, Ries, Draviam: "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells." in: Nature communications, Vol. 8, Issue 1, pp. 150, (2017) (PubMed).

Mo, Zhuang, Liu, Yao, Qin, Su, Zang, Wang, Zhang, Dou, Tian, Teng, Niu, Hill, Fang, Ding, Fu, Yao: "Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation." in: Nature chemical biology, Vol. 12, Issue 4, pp. 226-32, (2016) (PubMed).

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." in: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, (2016) (PubMed).

Asghar, Lajeunesse, Dulla, Combes, Thebault, Nigg, Elowe: "Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation." in: Nature communications, Vol. 6, pp. 8364, (2015) (PubMed).

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." in: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, (2014) (PubMed).

Carretero, Ruiz-Torres, Rodríguez-Corsino, Barthelemy, Losada: "Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres." in: The EMBO journal, Vol. 32, Issue 22, pp. 2938-49, (2014) (PubMed).

Nijenhuis, Vallardi, Teixeira, Kops, Saurin: "Negative feedback at kinetochores underlies a responsive spindle checkpoint signal." in: Nature cell biology, Vol. 16, Issue 12, pp. 1257-64, (2014) (PubMed).

Rivera, Ghenoiu, Rodríguez-Corsino, Mochida, Funabiki, Losada: "Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex." in: The EMBO journal, Vol. 31, Issue 6, pp. 1467-79, (2012) (PubMed).

Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." in: The Journal of cell biology, Vol. 191, Issue 5, pp. 923-31, (2011) (PubMed).

Wheatley, Henzing, Dodson, Khaled, Earnshaw: "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5655-60, (2004) (PubMed).
抗原

Aurora Kinase B (AURKB)

别名

AURKB (AURKB 产品)

背景

Background: Aurora Kinase B (Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase. Anti-AUROA B pT232 Antibody is useful for researchers interested in gene expression, DNA damage, cytokinesis, and transcription activities.

基因ID

9212, 83776600

UniProt

Q96GD4

途径

TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades