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Aurora Kinase B 抗体 (pThr232)

Name: Aurora Kinase B 抗体 (pThr232)
Brand: antibodies-online
SKU: ABIN129612
Availability: OutOfStock

AURKB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). (Aurora Kinase B 抗体 (pThr232))

Bufalin prevents Aurora A recruitment to mitotic centrosomes and Aurora B recruitment to unattached kinetochores(A) HeLa cells were synchronized by a single thymidine treatment, released in the presence or absence of bufalin (100 nM) for 9 h, and stained for phospho-Aurora A (Green), α-tubulin (Red) and DNA (Blue). (Aurora Kinase B 抗体 (pThr232))

IF (cc)

Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. (Aurora Kinase B 抗体 (pThr232))

IHC

High Aurora-B activity promotes KT attachment to MT-walls. (Aurora Kinase B 抗体 (pThr232))

IF (cc)

4 images

(18 references)

产品编号 ABIN129612

发货至: 中国

Datasheet as PDF

抗原 See all Aurora Kinase B (AURKB) 抗体

Aurora Kinase B (AURKB)
抗原表位
20

19

17

13

10

8

8

7

6

6

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 223-234, pThr232
适用
125

69

36

13

5

5

3

1

1

1

1

1

1
人
宿主
121

23

3

1
兔
Compatible Secondaries
克隆类型
109

40
多克隆
标记
83

11

8

7

5

5

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Aurora Kinase B antibody is un-conjugated
应用范围
93

66

43

36

17

14

14

13

8

6

4

3

3

3

2

2

1

1
Western Blotting (WB), ELISA

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein.

亚型

IgG
anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6H7 unconjugated

anti-Aurora Kinase B (AURKB) (AA 6-35) antibody
AURKB 适用: 人, 小鼠, 大鼠, 灵长类 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) antibody
AURKB 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Monoclonal 4E5 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6G8 unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rAURKB-1592 unconjugated Recombinant Antibody

anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 1-255) antibody
AURKB 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AURKB-1592 unconjugated

anti-Aurora Kinase B (AURKB) (pThr232) antibody
AURKB 适用: 人, 小鼠, 大鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Aurora Kinase B (AURKB) (AA 89-251) antibody
AURKB 适用: 人 WB 宿主: 小鼠 Monoclonal AURKB-1521 unconjugated

应用备注

This affinity-purified antibody has been tested for use in ELISA and by western blot. See below for specific protocol. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.

限制

仅限研究用
状态

Liquid

浓度

1.21 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Barroso-Vilares, Macedo, Reis, Warren, Compton, Logarinho: "Small-molecule inhibition of aging-associated chromosomal instability delays cellular senescence." in: EMBO reports, Vol. 21, Issue 5, pp. e49248, (2020) (PubMed).

Achuthankutty, Thakur, Haahr, Hoffmann, Drainas, Bizard, Weischenfeldt, Hickson, Mailand: "Regulation of ETAA1-mediated ATR activation couples DNA replication fidelity and genome stability." in: The Journal of cell biology, Vol. 218, Issue 12, pp. 3943-3953, (2020) (PubMed).

Novais-Cruz, Alba Abad, van IJcken, Galjart, Jeyaprakash, Maiato, Ferrás: "Mitotic progression, arrest, exit or death relies on centromere structural integrity, rather than de novo transcription." in: eLife, Vol. 7, (2019) (PubMed).

Liu, Kwon, Mannino, Yang, Renda, Khodjakov, Pellman: "Nuclear envelope assembly defects link mitotic errors to chromothripsis." in: Nature, Vol. 561, Issue 7724, pp. 551-555, (2019) (PubMed).

Xie, Lin, Wu, Tan, Cheng, Zhang: "Cardiac glycoside bufalin blocks cancer cell growth by inhibition of Aurora A and Aurora B activation via PI3K-Akt pathway." in: Oncotarget, Vol. 9, Issue 17, pp. 13783-13795, (2018) (PubMed).

Skowyra, Allan, Saurin, Clarke: "USP9X Limits Mitotic Checkpoint Complex Turnover to Strengthen the Spindle Assembly Checkpoint and Guard against Chromosomal Instability." in: Cell reports, Vol. 23, Issue 3, pp. 852-865, (2018) (PubMed).

Drpic, Almeida, Aguiar, Renda, Damas, Lewin, Larkin, Khodjakov, Maiato: "Chromosome Segregation Is Biased by Kinetochore Size." in: Current biology : CB, Vol. 28, Issue 9, pp. 1344-1356.e5, (2018) (PubMed).

de Cárcer, Venkateswaran, Salgueiro, El Bakkali, Somogyi, Rowald, Montañés, Sanclemente, Escobar, de Martino, McGranahan, Malumbres, Sotillo: "Plk1 overexpression induces chromosomal instability and suppresses tumor development." in: Nature communications, Vol. 9, Issue 1, pp. 3012, (2018) (PubMed).

Shrestha, Conti, Tamura, Braun, Ramalingam, Cieslinski, Ries, Draviam: "Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells." in: Nature communications, Vol. 8, Issue 1, pp. 150, (2017) (PubMed).

Mo, Zhuang, Liu, Yao, Qin, Su, Zang, Wang, Zhang, Dou, Tian, Teng, Niu, Hill, Fang, Ding, Fu, Yao: "Acetylation of Aurora B by TIP60 ensures accurate chromosomal segregation." in: Nature chemical biology, Vol. 12, Issue 4, pp. 226-32, (2016) (PubMed).

Redli, Gasic, Meraldi, Nigg, Santamaria: "The Ska complex promotes Aurora B activity to ensure chromosome biorientation." in: The Journal of cell biology, Vol. 215, Issue 1, pp. 77-93, (2016) (PubMed).

Asghar, Lajeunesse, Dulla, Combes, Thebault, Nigg, Elowe: "Bub1 autophosphorylation feeds back to regulate kinetochore docking and promote localized substrate phosphorylation." in: Nature communications, Vol. 6, pp. 8364, (2015) (PubMed).

Ohishi, Muramatsu, Yoshida, Seimiya: "TRF1 ensures the centromeric function of Aurora-B and proper chromosome segregation." in: Molecular and cellular biology, Vol. 34, Issue 13, pp. 2464-78, (2014) (PubMed).

Carretero, Ruiz-Torres, Rodríguez-Corsino, Barthelemy, Losada: "Pds5B is required for cohesion establishment and Aurora B accumulation at centromeres." in: The EMBO journal, Vol. 32, Issue 22, pp. 2938-49, (2014) (PubMed).

Nijenhuis, Vallardi, Teixeira, Kops, Saurin: "Negative feedback at kinetochores underlies a responsive spindle checkpoint signal." in: Nature cell biology, Vol. 16, Issue 12, pp. 1257-64, (2014) (PubMed).

Rivera, Ghenoiu, Rodríguez-Corsino, Mochida, Funabiki, Losada: "Xenopus Shugoshin 2 regulates the spindle assembly pathway mediated by the chromosomal passenger complex." in: The EMBO journal, Vol. 31, Issue 6, pp. 1467-79, (2012) (PubMed).

Mackay, Makise, Ullman: "Defects in nuclear pore assembly lead to activation of an Aurora B-mediated abscission checkpoint." in: The Journal of cell biology, Vol. 191, Issue 5, pp. 923-31, (2011) (PubMed).

Wheatley, Henzing, Dodson, Khaled, Earnshaw: "Aurora-B phosphorylation in vitro identifies a residue of survivin that is essential for its localization and binding to inner centromere protein (INCENP) in vivo." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 5655-60, (2004) (PubMed).
抗原

Aurora Kinase B (AURKB)

别名

Aurora B (AURKB 产品)

别名

AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, PPP1R48 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, AIRK2 antibody, AL022959 antibody, Aik2 antibody, Aim1 antibody, Ark2 antibody, STK-1 antibody, Stk12 antibody, Stk5 antibody, Aur B antibody, Aurora B antibody, Aurora-B antibody, CG6620 antibody, DmAIRK2 antibody, DmAurB antibody, DmAuroraB antibody, Dmel\\CG6620 antibody, IAL antibody, IAL1 antibody, Ial antibody, aurB antibody, aurora B antibody, ARK-2 antibody, XAIRK2 antibody, aik2 antibody, aim-1 antibody, aim1 antibody, airk2 antibody, ark2 antibody, ipl1 antibody, stk12 antibody, stk5 antibody, CHUNP6896 antibody, fa09g06 antibody, stka antibody, wu:fa09g06 antibody, aurkb antibody, AIRK2-B antibody, XAIRK2-B antibody, aurkb-a antibody, aurkb-b antibody, xAurora-B-B antibody, aurora kinase B antibody, aurora B antibody, aurora kinase B S homeolog antibody, AURKB antibody, Aurkb antibody, aurB antibody, aurkb antibody, aurkb.S antibody

背景

Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
Synonyms: AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody

基因ID

9212, 83776600

UniProt

Q96GD4

途径

TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades