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PRKDC 抗体 (pThr2609)

Name: PRKDC 抗体 (pThr2609)
Brand: antibodies-online
SKU: ABIN129603
Availability: OutOfStock

PRKDC 适用: 人 ELISA, WB, IP 宿主: 兔 Polyclonal unconjugated

Western blot using Affinity Purified anti-DNAPKcs antibody shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. (PRKDC 抗体 (pThr2609))

Western Blot results of Rabbit Anti-DNA Pkcs pT609 Antibody. (PRKDC 抗体 (pThr2609))

2 images

(5 references)

产品编号 ABIN129603

发货至: 中国

Datasheet as PDF

抗原 See all PRKDC 抗体

PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))
抗原表位
25

13

12

7

7

5

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr2609
适用
164

88

47

5

2

2

2

2

1
人
宿主
153

22

3

2
兔
Compatible Secondaries
克隆类型
150

30
多克隆
标记
92

11

10

7

5

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3
This PRKDC antibody is un-conjugated
应用范围
81

66

57

32

30

28

27

23

14

13

7

6

5

4

2

2

2

1
ELISA, Western Blotting (WB), Immunoprecipitation (IP)

交叉反应

Chimpanzee

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr 2609 of human DNA PKcs.

亚型

IgG

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anti-Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC) (AA 4075-4104), (C-Term) antibody
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应用备注

This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 460 kDa in size corresponding to DNA PKcs by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. Best western blotting results are seen when IP is performed prior to detection. This antibody detects an inducible signal at the correct height after DNA damage. However, the antibody may also detect an IR-inducible signal in cells lacking DNA PKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly. We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and is also phosphorylated in an IR-dependent manner. Similar results were seen with another phospho DNA PKcs antibody indicating that this result is general to antibodies to this phospho site, rather than specific to this antibody.

限制

仅限研究用
状态

Liquid

浓度

0.93 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Yoshikawa, Kashino, Ono, Watanabe: "Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities." in: Journal of radiation research, Vol. 50, Issue 2, pp. 151-60, (2009) (PubMed).

Wanner, Mayer, Kehlbach, Rodemann, Dittmann: "Activation of protein kinase Cepsilon stimulates DNA-repair via epidermal growth factor receptor nuclear accumulation." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 383-90, (2008) (PubMed).

Dittmann, Mayer, Kehlbach, Rodemann: "The radioprotector Bowman-Birk proteinase inhibitor stimulates DNA repair via epidermal growth factor receptor phosphorylation and nuclear transport." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 375-82, (2008) (PubMed).

Dittmann, Mayer, Wanner, Kehlbach, Rodemann: "The radioprotector O-phospho-tyrosine stimulates DNA-repair via epidermal growth factor receptor- and DNA-dependent kinase phosphorylation." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 84, Issue 3, pp. 328-34, (2007) (PubMed).

Dittmann, Mayer, Ohneseit, Raju, Andratschke, Milas, Rodemann: "Celecoxib induced tumor cell radiosensitization by inhibiting radiation induced nuclear EGFR transport and DNA-repair: a COX-2 independent mechanism." in: International journal of radiation oncology, biology, physics, Vol. 70, Issue 1, pp. 203-12, (2007) (PubMed).
抗原

PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

别名

DNA PKcs (PRKDC 产品)

别名

DNA-PKcs antibody, DNAPK antibody, DNPK1 antibody, HYRC antibody, HYRC1 antibody, XRCC7 antibody, p350 antibody, DNA-PKCS antibody, dna-pkcs antibody, dnapk antibody, dnpk1 antibody, hyrc antibody, hyrc1 antibody, prkdc-A antibody, xrcc7 antibody, PRKDC antibody, AI326420 antibody, AU019811 antibody, DNAPDcs antibody, DOXNPH antibody, dxnph antibody, p460 antibody, scid antibody, slip antibody, protein kinase, DNA-activated, catalytic polypeptide antibody, protein kinase, DNA-activated, catalytic polypeptide L homeolog antibody, DNA-dependent protein kinase catalytic subunit antibody, protein kinase, DNA activated, catalytic polypeptide antibody, PRKDC antibody, prkdc.L antibody, LOC100633457 antibody, Prkdc antibody

背景

DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and p460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide. Alternative splicing can occur for this protein to produce at least two isoforms.
Synonyms: DNA dependent protein kinase catalytic subunit antibody, DNA PK catalytic subunit antibody, DNAPK antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, HYRC 1 antibody

基因ID

5591, 13654237

UniProt

P78527

途径

DNA Damage Repair, Production of Molecular Mediator of Immune Response