Concentration Definition: by UV absorbance at 280 nm
免疫原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of human HAUSP protein.
USP7
适用: 人
IHC, ELISA, IF
宿主: 兔
Polyclonal
unconjugated
应用备注
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to HAUSP protein by western blotting in the appropriate cell lysate or extract.
限制
仅限研究用
状态
Liquid
浓度
0.98 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
抗原
USP7
(Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated) (USP7))
HAUSP (also known as deubiquitinating enzyme 7, herpes virus associated ubiquitin specific protease, TEF1, ubiquitin carboxyl terminal hydrolase 7, ubiquitin specific protease 7, ubiquitin thiolesterase 7, and USP7) is a novel p53 interacting protein. HAUSP was identified by mass spectrometry of affinity purified p53 associated factors by Li et al. HAUSP strongly stabilizes p53, even in the presence of excess MDM2, and also induces p53-dependent cell growth repression and apoptosis. HAUSP has an intrinsic enzymatic activity that specifically de-ubiquitinates p53 both in vivo and in vitro. Expression of a catalytically inactive point mutation of HAUSP in cells increased the levels of p53 ubiquitination and also destabilized p53. Li et al concluded that their findings revealed an important mechanism by which p53 can be stabilized by direct de-ubiquitination and also implied that HAUSP may function as a tumor suppressor in vivo through the stabilization of p53. Synonyms: Deubiquitinating enzyme 7 antibody, herpes virus associated antibody, Herpes virus associated ubiquitin specific protease antibody, TEF 1 antibody, tef-1 antibody, TEF1 antibody, Ubiquitin carboxyl terminal hydrolase 7 antibody