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CBX4 抗体 (AA 90-115)

Rockland 600-401-478 CBX4 适用: 人 WB, ELISA, IF, IP, FM 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129594
Supplier Product No.: 600-401-478
发货至: 中国
  • 抗原 See all CBX4 抗体
    CBX4 (Chromobox Homolog 4 (CBX4))
    抗原表位
    • 15
    • 8
    • 8
    • 6
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 90-115
    适用
    • 62
    • 32
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    宿主
    • 60
    • 2
    克隆类型
    • 61
    • 1
    多克隆
    标记
    • 24
    • 5
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CBX4 antibody is un-conjugated
    应用范围
    • 49
    • 31
    • 15
    • 13
    • 13
    • 11
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
    Supplier Product No.
    600-401-478
    Supplier
    Rockland
    原理
    PC2 Antibody
    交叉反应 (详细)
    Reactivity occurs against human PC2 protein.
    产品特性
    Synonyms: rabbit anti-PC2 antibody, E3 SUMO-protein ligase CBX4, Chromobox protein homolog 4, Polycomb 2 homolog, Pc2, hPc2, CBX4
    纯化方法
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 90-115 of Human PC2 protein.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product CBX4 Primary Antibody
  • 应用备注

    Application Note: Anti-PC2 antibody has been tested for use in ELISA, WB, and IF. This affinity purified antibody is useful in immunofluorescence staining of cultured cells. In immunofluorescence, this antibody detects the expected discrete nuclear structure that is termed the PcG body, corresponding to the known localization of PC2. This affinity purified antibody is useful in western blotting using transfected cell lysates. Dilutions for western blotting represent a starting point dilution and further optimization may be required.  The antibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). The antibody has been successfully used to detect FLAG-tagged transfected hPC2 (see figure).  It detects a weak band that probably corresponds to endogenous PC2, however, a strong secondary band is also seen at 50kD in all cell lines thus far tested. This suggests that the antibody may also react with another highly expressed ubiquitous protein.  Reactivity in other immunoassays is unknown. 

    Western Blot Dilution: 1:1,000 - 1:4,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:10,000 - 1:40,000

    IF Microscopy Dilution: 1:100 - 1:400

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.36 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    CBX4 (Chromobox Homolog 4 (CBX4))
    别名
    CBX4 (CBX4 产品)
    背景
    Background: PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to depression of proto-oncogene transcription and subsequently to cellular transformation.  Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.
    基因ID
    8535
    UniProt
    O00257
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