Concentration Definition: by UV absorbance at 280 nm
免疫原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 40-49 of human UBP43 protein.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to UBP43 by western blotting in the appropriate cell lysate or extract.
限制
仅限研究用
状态
Liquid
浓度
0.9 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. Synonyms: 3 kDa ISG15 specific protease antibody, EC 3.1.2. antibody, hUBP43 antibody, Interferon Stimulated Gene 43 kD antibody, ISG15 Specific Processing Protease antibody, ISG43 antibody, Ubiquitin Specific Peptidase 18 antibody