USP18 抗体 (AA 25-50)
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- 抗原 See all USP18 抗体
- USP18 (Ubiquitin Specific Peptidase 18 (USP18))
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抗原表位
- AA 25-50
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This USP18 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-476
- Supplier
- Rockland
- 原理
- UBP43 Antibody
- 交叉反应 (详细)
- This affinity-purified antibody is directed against human UBP43 protein.
- 产品特性
- Synonyms: rabbit anti-UBP43 antibody, rabbit anti-USP18 antibody, rabbit anti-ISG43 antibody, 43 kDa ISG15 specific protease antibody, hUBP43 antibody, Interferon Stimulated Gene 43 kD antibody, ISG15 Specific Processing Protease antibody, ISG43 antibody, Ubiquitin Specific Peptidase 18 antibody, Ubl carboxyl-terminal hydrolase 18, Ubl thioesterase 18
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product USP18 Primary Antibody
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to UBP43 by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.9 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- USP18 (Ubiquitin Specific Peptidase 18 (USP18))
- 别名
- USP18 (USP18 产品)
- 背景
- Background: UBP43 (also known as ISG43, Ubl thiolesterase 18, ISG15-specific processing protease, 43 kDa ISG15-specific protease, ubiquitin specific protease 18 or USP18) is a member of the deubiquitinating protease family of enzymes, which removes ubiquitin adducts from a broad range of protein substrates. Deletion of the UBP43 gene in mouse leads to a massive increase of ISG15 conjugates in tissues indicating that UBP43 is a major ISG15-specific protease. UBP43 is the first ISG15-specific protease reported. Both ISG15 and UBP43 genes are known to be strongly induced by interferon, genotoxic stress, and viral infection. Thus UBP43 may be necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
- 基因ID
- 11274, 32313610
- UniProt
- Q9UMW8
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