Aurora Kinase B 抗体 (AA 225-250)
(1 reference)
Quick Overview for Aurora Kinase B 抗体 (AA 225-250) (ABIN129590)
抗原
See all Aurora Kinase B (AURKB) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 225-250
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Supplier Product No.
- 600-401-465
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Supplier
- Rockland
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原理
- AURORA KINASE B Antibody
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交叉反应 （详细）
- Reactivity occurs against human Aurora Kinase B protein.
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产品特性
- Synonyms: rabbit anti-Aurora B Antibody, AIK2 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, AURKB antibody, Aurora 1 antibody, Aurora and Ipl1 like midbody associated protein 1 antibody
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纯化方法
- Aurora B antibody is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
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过滤
- Sterile filtered
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免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 225-250 of Human Aurora Kinase B protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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anti-Aurora Kinase B (AURKB) (AA 1-90) antibody
AURKB 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 6H7 unconjugated
anti-Aurora Kinase B (AURKB) (Internal Region) antibodyAURKB 适用: 人, 猴 WB, IHC, ELISA, FM 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 6-35) antibodyAURKB 适用: 人, 小鼠, 大鼠, 灵长类 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) antibodyAURKB 适用: 人 WB, IHC, ELISA, IP 宿主: 兔 Monoclonal 4E5 unconjugated Recombinant Antibody
anti-Aurora Kinase B (AURKB) (AA 1-90) antibodyAURKB 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 6G8 unconjugated
anti-Aurora Kinase B (AURKB) (AA 89-251) antibodyAURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rAURKB-1592 unconjugated Recombinant Antibody
anti-Aurora Kinase B (AURKB) (pThr232) antibodyAURKB 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 1-255) antibodyAURKB 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (pThr232) antibodyAURKB 适用: 人, 小鼠, 大鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Aurora Kinase B (AURKB) (AA 89-251) antibodyAURKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal AURKB-1592 unconjugated
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应用备注
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Application Note: Anti-Aurora B is affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 39 kDa in size corresponding to Aurora Kinase B by western blotting in the appropriate cell lysate or extract. Human brain tissue lysate or HeLa cell lysate can be used as a positive control.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: User Optimized
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.39 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store Anti-Aurora B at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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: "A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4130-40, (2008) (PubMed).
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: "A mitotic GlcNAcylation/phosphorylation signaling complex alters the posttranslational state of the cytoskeletal protein vimentin." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4130-40, (2008) (PubMed).
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- Aurora Kinase B (AURKB)
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别名
- AURKB
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背景
- Background: Aurora Kinase B (also known as Aurora- and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
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基因ID
- 9212, 83776600
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UniProt
- Q96GD4
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途径
- TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades
抗原
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