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DNMT3L 抗体 (AA 150-175)

Rockland 600-401-454 TRDMT1 适用: 人 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
Rockland
产品编号 ABIN129584
Supplier Product No.: 600-401-454
发货至: 中国
  • 抗原 See all DNMT3L (TRDMT1) 抗体
    DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
    抗原表位
    • 15
    • 8
    • 7
    • 7
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 150-175
    适用
    • 66
    • 17
    • 2
    • 1
    宿主
    • 66
    • 5
    克隆类型
    • 68
    • 3
    多克隆
    标记
    • 32
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This DNMT3L antibody is un-conjugated
    应用范围
    • 58
    • 32
    • 14
    • 13
    • 13
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Supplier Product No.
    600-401-454
    Supplier
    Rockland
    原理
    DNMT3L Antibody
    交叉反应 (详细)
    This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76 % identity with mouse.
    产品特性
    Synonyms: rabbit anti-DNMT3L antibody, DNA (cytosine-5)-methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L
    纯化方法
    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-)-methyltransferase 3-like). DNMT3L is a nuclear protein with similarity to DNA methyltransferases.

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product TRDMT1 Primary Antibody
  • 应用备注

    Immunohistochemistry Dilution: 1:500

    Application Note: Anti-DNMT3L antibody has been tested for use in ELISA against the immunizing peptide.  This antibody has been tested in immunohistochemistry and western blot. This antibody works for western blotting only following immunoprecipitation.  The expected molecular weight of human DNMT3L is 43.6 kDa. Reactivity in other immunoassays is unknown.

    Western Blot Dilution: 1:500

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:1000 - 1:3000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.11 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))
    别名
    DNMT3L (TRDMT1 产品)
    背景
    Background: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
    基因ID
    29947
    NCBI登录号
    NP_787063
    UniProt
    Q9UJW3
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