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PLK1 抗体 (pThr210)

Name: PLK1 抗体 (pThr210)
Brand: antibodies-online
SKU: ABIN129581
Availability: OutOfStock

PLK1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. (PLK1 抗体 (pThr210))

Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human colon carcinoma tumor tissue. (PLK1 抗体 (pThr210))

IHC

Western blot analysis is shown using Affinity Purified anti-Plk-1 pT210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). (PLK1 抗体 (pThr210))

Affinity Purified Plk1 pT210 was used at a 1:200 dilution to detect Plk1 by immunohistochemistry in human breast carcinoma tumor tissue. (PLK1 抗体 (pThr210))

IHC

4 images

(1 reference)

产品编号 ABIN129581

发货至: 中国

Datasheet as PDF

抗原 See all PLK1 抗体

PLK1 (Polo-Like Kinase 1 (PLK1))
抗原表位
22

17

15

12

8

5

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr210
适用
122

45

41

7

7

5

5

5

5

3

3

3

1

1

1

1
人, 小鼠
宿主
98

23

2

1
兔
Compatible Secondaries
克隆类型
101

23
多克隆
标记
74

5

5

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This PLK1 antibody is un-conjugated
应用范围
99

46

32

28

27

20

19

16

7

5

4

4

3

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

原理

PLK1 phospho T210 Antibody

交叉反应（详细）

Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein.

产品特性

Synonyms: rabbit anti-PLK1 pT210 antibody, Serine/threonine-protein kinase PLK1, rabbit anti-polio like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, PLK-1, PLK 1, STPK13

纯化方法

This antibody was affinity purified.

过滤

Sterile filtered

免疫原

Immunogen: Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein.
Immunogen Type: Conjugated Peptide

亚型

IgG

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anti-Polo-Like Kinase 1 (PLK1) antibody
PLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated

应用备注

Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: This affinity-purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 68 kDa in size corresponding to Plk-1 by western blotting in the appropriate cell lysate or extract. This antibody is positive by IHC on kidney, liver, cancer, thyroid and lymphocyte tissue.
Western Blot Dilution: 1:200 - 1:2,000
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Adler, Müller, Rached, Dekant, Mally: "Modulation of key regulators of mitosis linked to chromosomal instability is an early event in ochratoxin A carcinogenicity." in: Carcinogenesis, Vol. 30, Issue 4, pp. 711-9, (2009) (PubMed).
抗原

PLK1 (Polo-Like Kinase 1 (PLK1))

别名

PLK1 (PLK1 产品)

背景

Background: Polo-like Kinase pT210 Antibody detects phosphorylated Plk1 protein. Polo-like kinase 1, also known as Plk-1, serine-threonine protein kinase 13 and STPK13, may be required for cell division and have a role during G₁ or S phase and is associated with the phosphorylation of cyclin B₁. Phosphorylation of Plk-1 on T210 contributes to proper mitotic progression. Plk-1 has a nuclear localization and accumulates to a maximum during the G₂ and M phases, declines to a nearly undetectable level following mitosis and throughout G₁ phase, and then begins to accumulate again during S phase. Plk-1 is a member of the Ser/Thr protein kinase family and the CDC5/Polo subfamily and contains 2 POLO box domains.

基因ID

5347, 21359873

UniProt

P53350

途径

Cell Division Cycle, M Phase