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RFP 抗体

Name: RFP 抗体
Brand: antibodies-online
SKU: ABIN129578
Availability: OutOfStock
Rating: 5 (1 reviews)

RFP 适用: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867) (RFP 抗体)

Western Blot of Rabbit anti-RFP antibody. (RFP 抗体)

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody. (RFP 抗体)

Immunohistochemistry of Anti-RFP Antibody. (RFP 抗体)

IHC

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody. (RFP 抗体)

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. (RFP 抗体)

ELISA

Western Blot of RFP Antibody Pre-Absorbed. (RFP 抗体)

12 images

(356 references)

(1 validation)

产品编号 ABIN129578

发货至: 中国

Datasheet as PDF

Key Features
- Frequently cited in scientific publications with 300+ citations
- Validated inhouse for relevant applications
- Extensive validation report for IF provided by one of your peers
- Designed to detect RFP and its variants
抗原 See all RFP 抗体

RFP (Red Fluorescent Protein (RFP))

适用

Discosoma
宿主
21

14

9

5

3
兔
Compatible Secondaries
克隆类型
30

22
多克隆
标记
18

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RFP antibody is un-conjugated
应用范围
26

20

15

11

9

6

5

5

2

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Polyclonal RFP antibody is designed to detect RFP and its variants.

交叉反应（详细）

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).
No reaction was observed against Human, Mouse or Rat serum proteins.

纯化方法

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

过滤

Sterile filtered

免疫原

The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein

亚型

IgG

Product Specific Information

What can the RFP Antibody ABIN129578 be used for?

This polyclonal RFP Antibody detects RFP. The RFP Antibody has been validated for various applications and can be used for the detection of RFP and derivatives by Western Blotting, ELISA, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Immunohistochemistry (Frozen Sections), Immunohistochemistry (Paraffin-embedded Sections).

What validation data is available for this RFP Antibody?

The Primary Antibody is referenced in 356 publications and is characterized by a proven, very high reliability. It has currently 11 product images that show its performance in a variety of applications. The product is currently available in 10 μL, 100 μL quantities. RFP Antibody for the detection of RFP and derivatives.
anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633

anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488

anti-Red Fluorescent Protein (RFP) antibody (HRP)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP

anti-Red Fluorescent Protein (RFP) antibody (DyLight 405)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 405

应用备注

Suggested dilutions:
ELISA: 1:28,700 - 1:48,700
IF: 1:200 - 1:2,000
FACS: 1:200 - 1:2,000
WB: 1:1,000 - 1:5,000
IHC: 1:200 - 1:2,000
IP: User Optimized

说明

This RFP antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen.
Optimal titers for applications should be determined by the researcher.

限制

仅限研究用
生效 #101103 (Immunofluorescence)

by

Centre d'Immunologie de Marseille-Luminy

No.

#101103

日期

2017.03.07

抗原

RFP

Lot Number

34945

Method validated

Immunofluorescence

Positive Control

CX3CL1mcherry⁺ murine thymus

Negative Control

CX3CL1 WT murine thymus

Notes

Passed. The RFP antibody ABIN129578 specifically labels RFP⁺ cells in CX3CL1mcherry⁺ murine thymus in immunofluorescence, consistent with the expression pattern of CX3CL1mcherry.

Validation Images

CX3CL1 positive cells in CX3CL1mcherry murine thymus (A, mCherry) were stained with ABIN1043867 and an AF647 conjugated secondary antibody (B) or with the secondary antibody alone (C). CX3CL1 positive cells in CX3CL1 wt murine thymus were stained with ABIN1043867 and the AF647 conjugated antibody (D). Scale bar 100?m, 20x magnification, 1000 resolution
Full Methods
Primary Antibody

ABIN1043867

Secondary Antibody

donkey Fab'2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)

Full Protocol

Harvest thymus from 7 weeks old mice in 1X Dulbecco's phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
Fix mouse thymus in antigen fix (Diapath, P0014) for 2h at 4?C.
Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4?C.
Dehydrate tissue in 30% sucrose solution at 4?C ON.
Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80?C.
Cut blocks into 25?m sections using a cryostat (Leica, CM3050 S).
Transfer sections to a slide.
Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
Place slide in a humidified chamber and rehydrate sections in 0.1 M TrisHCl pH7.4 for 10min at RT.
Gently remove buffer by tapping slide.
Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, lot 015K0039) and 0.5% BSA, for 20min at RT.
Incubate sections with primary RFP antibody (Red Fluorescent Protein) (AA 234) (antibodies-online, ABIN129578, lot 34945) diluted 1:1000 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
Incubate a no primary antibody negative control in parallel in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA.
Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
Incubate sections with secondary donkey Fab'2 anti-rabbit AF647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:300 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
Add approximately 10µL of Slowfade Gold antifade reagent (ThermoFisher Scientific, S36937, lot 1226836) for each section and mount cover slip.
Image acquisition on an LSM 880 (Zeiss), 20x magnification, 1000 resolution.

Experimental Notes
状态

Liquid

浓度

1.16 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid cycles of freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Simpson Ragdale, Clements, Tang, Deltcheva, Andreassi, Lai, Chang, Pandrea, Andrew, Game, Uddin, Ellis, Enver, Riccio, Marguerat, Parrinello: "Injury primes mutation-bearing astrocytes for dedifferentiation in later life." in: Current biology : CB, (2023) (PubMed).

Amodeo, Davies, Martinez-Segura, Clements, Ragdale, Bailey, Dos Santos, MacRae, Mokochinski, Kramer, Garcia-Diaz, Gould, Marguerat, Parrinello: "Diet suppresses glioblastoma initiation in mice by maintaining quiescence of mutation-bearing neural stem cells." in: Developmental cell, (2023) (PubMed).

Scholz, Dahse, Kemkemer, Bormann, Auger, Vieira Contreras, Ernst, Staake, Körner, Buhlan, Meyer-Mölck, Chung, Blanco-Redondo, Klose, Jarboui, Ljaschenko, Bigl, Langenhan: "Molecular sensing of mechano- and ligand-dependent adhesion GPCR dissociation." in: Nature, Vol. 615, Issue 7954, pp. 945-953, (2023) (PubMed).

Middelkamp, Ruck, Krisp, Sumisławski, Mohammadi, Dottermusch, Meister, Küster, Schlüter, Windhorst, Neumann: "Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine density." in: Acta neuropathologica communications, Vol. 9, Issue 1, pp. 185, (2022) (PubMed).

Pouchelon, Vergara, McMahon, Gorissen, Lin, Vormstein-Schneider, Niehaus, Burbridge, Wester, Sherer, Fernandez-Otero, Allaway, Pelkey, Chittajallu, McBain, Fan, Nasse, Wildenberg, Fishell et al.: "A versatile viral toolkit for functional discovery in the nervous system. ..." in: Cell reports methods, Vol. 2, Issue 6, pp. 100225, (2022) (PubMed).

Dumoulin, Zuñiga, Stoeckli: "Axon guidance at the spinal cord midline-A live imaging perspective." in: The Journal of comparative neurology, (2021) (PubMed).

Park, Lofton, Li, Rasin: "Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs." in: Cells, Vol. 10, Issue 2, (2021) (PubMed).

Dottermusch, Sumisławski, Krevet, Middelkamp, Voß, Siebels, Bartsch, Sotlar, Meyer, Frank, Korshunov, Glatzel, Schüller, Neumann: "Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma." in: Oncogenesis, Vol. 10, Issue 11, pp. 78, (2021) (PubMed).

Brooks, Clements, Burden, Kocher, Richards, Devesa, Zakka, Woodberry, Ellis, Jaunmuktane, Brandner, Morrison, Pollard, Dirks, Marguerat, Parrinello: "The white matter is a pro-differentiative niche for glioblastoma." in: Nature communications, Vol. 12, Issue 1, pp. 2184, (2021) (PubMed).

Dannhäuser, Lux, Hu, Selcho, Chen, Ehmann, Sachidanandan, Stopp, Pauls, Pawlak, Langenhan, Soba, Rittner, Kittel: "Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination." in: eLife, Vol. 9, (2021) (PubMed).

Bellomo, Mondor, Spinelli, Lagueyrie, Stewart, Brouilly, Malissen, Clatworthy, Bajénoff: "Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages." in: Immunity, Vol. 53, Issue 1, pp. 127-142.e7, (2020) (PubMed).

Eun, Hong, Jeong, Park, Hwang, Jeong, Choi, Olsson, Hwang, Hyun, Kim: "Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer." in: PLoS ONE, Vol. 15, Issue 6, pp. e0233784, (2020) (PubMed).

Fu, Wang, Chouairi, Rose, Abetov, Miller, Yamulla, Schimenti, Flesken-Nikitin, Nikitin: "Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells." in: Nature communications, Vol. 11, Issue 1, pp. 84, (2020) (PubMed).

Schaub, Rose, Frasch: "Yorkie and JNK revert syncytial muscles into myoblasts during Org-1-dependent lineage reprogramming." in: The Journal of cell biology, Vol. 218, Issue 11, pp. 3572-3582, (2020) (PubMed).

Green, Cavey, Médina Caturegli, Aigouy, Gompel, Prudhomme: "Evolution of Ovipositor Length in Drosophila suzukii Is Driven by Enhanced Cell Size Expansion and Anisotropic Tissue Reorganization." in: Current biology : CB, Vol. 29, Issue 12, pp. 2075-2082.e6, (2020) (PubMed).

Henschke, Pakan: "Disynaptic cerebrocerebellar pathways originating from multiple functionally distinct cortical areas." in: eLife, Vol. 9, (2020) (PubMed).

Zhou, Zhong, Peng, Liu, Ding, Sun, Ma, Liu, Chen, Wu, Wang: "Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus." in: Nature communications, Vol. 11, Issue 1, pp. 4063, (2020) (PubMed).

Klouda, Condon, Hao, Tian, Lvova, Chakraborty, Nicolls, Zhou, Raby, Yuan: "From 2D to 3D: Promising Advances in Imaging Lung Structure." in: Frontiers in medicine, Vol. 7, pp. 343, (2020) (PubMed).

Ricci, Tycksen, Celik, Belle, Fontana, Civitelli, Faccio: "Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics." in: eLife, Vol. 9, (2020) (PubMed).

Kennedy, Rinker, Broadie: "Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model." in: BMC biology, Vol. 18, Issue 1, pp. 94, (2020) (PubMed).

show more references
抗原

RFP (Red Fluorescent Protein (RFP))

别名

RFP (RFP 产品)

背景

Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure.