CHEK2 抗体 (pThr68)
(1 reference)
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- 抗原 See all CHEK2 抗体
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
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抗原表位
- pThr68
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHEK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Supplier Product No.
- 600-401-280
- Supplier
- Rockland
- 原理
- CHK2 phospho T68 Antibody
- 交叉反应 （详细）
- This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue.
- 产品特性
- Synonyms: rabbit anti-CHK2 pT68 Antibody, Serine/threonine-protein kinase Chk2, CHK-2, CHK 2, CDS1, RAD53, CHEK2, Checkpoint kinase 2, Hucds1
- 纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 50-75 of Human CHK2.
Immunogen Type: Conjugated Peptide
- 亚型
- IgG
- Top Product
- Discover our top product CHEK2 Primary Antibody
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anti-Checkpoint Kinase 2 (CHEK2) (pThr387) antibody
CHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-220) antibodyCHEK2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibodyCHEK2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (AA 1-543) antibodyCHEK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B7 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pSer516) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) (pThr383) antibodyCHEK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 猴, 犬 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 10G7 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 犬 WB, IHC, IF, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 5C4 unconjugated
anti-Checkpoint Kinase 2 (CHEK2) antibodyCHEK2 适用: 人, 犬 WB, IHC, IHC (p), IP, FACS 宿主: 小鼠 Monoclonal 2H5 unconjugated
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- 应用备注
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Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 61 kDa in size corresponding to CHK2 by western blotting in the appropriate cell lysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated form of the immunizing peptide. This antibody is phospho specific for pT68 of CHK2.
Western Blot Dilution: 1:200 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:70,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." in: Cancer research, Vol. 68, Issue 7, pp. 2523-9, (2008) (PubMed).
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: "Effect of caffeine on the ATR/Chk1 pathway in the epidermis of UVB-irradiated mice." in: Cancer research, Vol. 68, Issue 7, pp. 2523-9, (2008) (PubMed).
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- 抗原
- CHEK2 (Checkpoint Kinase 2 (CHEK2))
- 别名
- CHEK2 (CHEK2 产品)
- 背景
- Background: CHK2 (also known as CHEK2, Protein kinase CHK2 isoform a, and checkpoint-like protein) is a serine/ threonine-protein kinase involved in the control of cell cycle checkpoints and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.
- 基因ID
- 11200
- NCBI登录号
- NP_001005735
- UniProt
- O96017
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle
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