NCS1 抗体
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- 抗原 See all NCS1 抗体
- NCS1 (Neuronal Calcium Sensor 1 (NCS1))
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NCS1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Multiplex Assay (MA), Fluorescence Microscopy (FM)
- Supplier Product No.
- 600-401-216
- Supplier
- Rockland
- 原理
- Frequenin Antibody
- 交叉反应 (详细)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 产品特性
- Synonyms: rabbit anti-FREQUENIN antibody, NCS-1, FLUP, FREQ, Neuronal calcium sensor 1, frequenin homolog antibody, Frequenin-like protein antibody, NCS 1 antibody, NCS1 antibody
- 纯化方法
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Frequenin (recombinant) coupled to agarose beads.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Frequenin (recombinant from Mouse with extensive post-translational modifications)
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product NCS1 Primary Antibody
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- 应用备注
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Application Note: This product was assayed by immunoblot and found to be reactive against Frequenin at a dilution of 1:5000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302. Anti-Frequenin is suitable for the detection by immunoblot of human, mouse and rat Frequenin. Anti-Frequenin has also been tested for use in IF using Hippocampal neurons of 17 day old NMRI mice.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:10,000 - 1:50,000
IF Microscopy Dilution: 1:100-1:2500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 2.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- NCS1 (Neuronal Calcium Sensor 1 (NCS1))
- 别名
- Ncs1 (NCS1 产品)
- 背景
- Background: Frequenin, or Neuronal calcium sensor, is a regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. It directly regulates GRK1 (RHOK), but not GRK2 to GRK5. It can substitute for calmodulin and it stimulates PI4KB kinase activity. It is involved in long-term synaptic plasticity through its interaction with PICK1. Ncs1 may also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
- 基因ID
- 14299
- NCBI登录号
- NP_062655
- UniProt
- Q8BNY6
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