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GFP 抗体 (AA 246) (Texas Red (TR))

Name: GFP 抗体 (AA 246) (Texas Red (TR))
Brand: antibodies-online
SKU: ABIN129569
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 Texas Red (TR)

Dot Blot results of Mouse Anti-GFP Antibody Texas Conjugated. (GFP 抗体 (AA 246) (Texas Red (TR)))

1 image

产品编号 ABIN129569

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
15

15

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1

1
AA 246
适用
175

15

11

9
Aequorea victoria
宿主
63

62

35

15

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
115

71
单克隆
标记
82

13

11

10

4

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Texas Red (TR)
应用范围
149

75

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA

交叉反应

recombinant GFP (rGFP), enhanced GFP (eGFP)

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

克隆位点

9F9-F9

亚型

IgG

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应用备注

Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

限制

仅限研究用
状态

Lyophilized

溶解方式

Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.