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GFP 抗体

Name: GFP 抗体
SKU: ABIN129564
Availability: OutOfStock

Rockland 600-301-215 GFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FACS, FM, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

Western Blot of Mouse anti-GFP antibody. (GFP 抗体)

SDS PAGE of Mouse anti-GFP antibody. (GFP 抗体)

SDS

Western Blot of monoclonal anti-GFP antibody. (GFP 抗体)

Mouse monoclonal Anti-GFP antibody is able to detect positive cell population in cytoflow analysis. (GFP 抗体)

FACS

4 images

(17 references)

产品编号 ABIN129564

Supplier Product No.: 600-301-215

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
适用
165

15

11

9
Aequorea victoria
宿主
62

56

34

13

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
110

67
单克隆
标记
78

11

10

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
142

73

47

40

26

25

25

21

16

13

13

13

12

11

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)

Supplier Product No.

600-301-215

Supplier

Rockland

原理

GFP Monoclonal Antibody

交叉反应（详细）

Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum.

产品特性

Synonyms: mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

纯化方法

GFP Monoclonal Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography.

过滤

Sterile filtered

免疫原

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Recombinant Protein

克隆位点

9F9-F9

亚型

IgG1 kappa

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应用备注

Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in ELISA, IP, and WB and suitable in FACS, IHC, IF. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with Rockland's polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP (code # S000-03) or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
Western Blot Dilution: 1:3,000 - 1:30,000
ELISA Dilution: 1:10,000 - 1:30,000
IF Microscopy Dilution: User Optimized
Other: User Optimized

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store mouse anti-GFP at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
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Santos, Damásio, Kun-Rodrigues, Barbot, Sequeiros, Brás, Alonso, Guerreiro: "Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype." in: Journal of clinical medicine, Vol. 9, Issue 4, (2020) (PubMed).

Dohmen, Krieg, Agalaridis, Zhu, Shehata, Pfeiffenberger, Amelang, Bütepage, Buerova, Pfaff, Chanda, Geley, Preisinger, Sakamoto, Lüscher, Neumann, Vervoorts: "AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy." in: Nature communications, Vol. 11, Issue 1, pp. 1032, (2020) (PubMed).

Santos, Morais, Pereira, Sequeiros, Alonso: "Parkin truncating variants result in a loss-of-function phenotype." in: Scientific reports, Vol. 9, Issue 1, pp. 16150, (2019) (PubMed).

Khlghatyan, Evstratova, Chamberland, Marakhovskaia, Bahremand, Toth, Beaulieu: "Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3β Are Modulators of Anxiety and Glutamatergic Neurotransmission." in: Frontiers in molecular neuroscience, Vol. 11, pp. 119, (2018) (PubMed).

Terrell, Wu, Tsao, Barber, Servinsky, Payne, Bentley: "Nano-guided cell networks as conveyors of molecular communication." in: Nature communications, Vol. 6, pp. 8500, (2016) (PubMed).

Moerdyk-Schauwecker, Shah, Murphy, Hastie, Mukherjee, Grdzelishvili: "Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling." in: Virology, Vol. 436, Issue 1, pp. 221-34, (2013) (PubMed).

Feijs, Kleine, Braczynski, Forst, Herzog, Verheugd, Linzen, Kremmer, Lüscher: "ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation." in: Cell communication and signaling : CCS, Vol. 11, Issue 1, pp. 5, (2013) (PubMed).

Hastie, Besmer, Shah, Murphy, Moerdyk-Schauwecker, Molestina, Roy, Curry, Mukherjee, Grdzelishvili: "Oncolytic vesicular stomatitis virus in an immunocompetent model of MUC1-positive or MUC1-null pancreatic ductal adenocarcinoma." in: Journal of virology, Vol. 87, Issue 18, pp. 10283-94, (2013) (PubMed).

Forst, Karlberg, Herzog, Thorsell, Gross, Feijs, Verheugd, Kursula, Nijmeijer, Kremmer, Kleine, Ladurner, Schüler, Lüscher: "Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).

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Jesse, Koenig, Ellenrieder, Menke: "Lef-1 isoforms regulate different target genes and reduce cellular adhesion." in: International journal of cancer, Vol. 126, Issue 5, pp. 1109-20, (2010) (PubMed).

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抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

背景

Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt

P42212