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GFP 抗体 (AA 246)

Name: GFP 抗体 (AA 246)
Brand: antibodies-online
SKU: ABIN129564
Availability: OutOfStock

GFP 适用: Aequorea victoria WB, ELISA 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated

Western Blot of Mouse anti-GFP antibody. (GFP 抗体 (AA 246))

SDS PAGE of Mouse anti-GFP antibody. (GFP 抗体 (AA 246))

SDS

Western Blot of monoclonal anti-GFP antibody. (GFP 抗体 (AA 246))

Mouse monoclonal Anti-GFP antibody is able to detect positive cell population in cytoflow analysis. (GFP 抗体 (AA 246))

FACS

4 images

(17 references)

产品编号 ABIN129564

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))
抗原表位
15

15

10

8

4

4

3

3

2

2

2

1

1

1

1

1

1

1
AA 246
适用
174

15

11

9
Aequorea victoria
宿主
63

62

35

14

9

5

1

1
小鼠
Compatible Secondaries
克隆类型
114

71
单克隆
标记
81

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is un-conjugated
应用范围
148

74

46

33

25

25

25

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA

交叉反应

recombinant GFP (rGFP), enhanced GFP (eGFP)

产品特性

Concentration Definition: by UV absorbance at 280 nm

免疫原

The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
Immunogentype:Recombinant

克隆位点

9F9-F9

亚型

IgG

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应用备注

Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C
Žaja, Aydin, Lippok, Feederle, Lüscher, Feijs: "Comparative analysis of MACROD1, MACROD2 and TARG1 expression, localisation and interactome." in: Scientific reports, Vol. 10, Issue 1, pp. 8286, (2020) (PubMed).

Santos, Damásio, Kun-Rodrigues, Barbot, Sequeiros, Brás, Alonso, Guerreiro: "Novel MAG Variant Causes Cerebellar Ataxia with Oculomotor Apraxia: Molecular Basis and Expanded Clinical Phenotype." in: Journal of clinical medicine, Vol. 9, Issue 4, (2020) (PubMed).

Dohmen, Krieg, Agalaridis, Zhu, Shehata, Pfeiffenberger, Amelang, Bütepage, Buerova, Pfaff, Chanda, Geley, Preisinger, Sakamoto, Lüscher, Neumann, Vervoorts: "AMPK-dependent activation of the Cyclin Y/CDK16 complex controls autophagy." in: Nature communications, Vol. 11, Issue 1, pp. 1032, (2020) (PubMed).

Santos, Morais, Pereira, Sequeiros, Alonso: "Parkin truncating variants result in a loss-of-function phenotype." in: Scientific reports, Vol. 9, Issue 1, pp. 16150, (2019) (PubMed).

Khlghatyan, Evstratova, Chamberland, Marakhovskaia, Bahremand, Toth, Beaulieu: "Mental Illnesses-Associated Fxr1 and Its Negative Regulator Gsk3β Are Modulators of Anxiety and Glutamatergic Neurotransmission." in: Frontiers in molecular neuroscience, Vol. 11, pp. 119, (2018) (PubMed).

Terrell, Wu, Tsao, Barber, Servinsky, Payne, Bentley: "Nano-guided cell networks as conveyors of molecular communication." in: Nature communications, Vol. 6, pp. 8500, (2016) (PubMed).

Moerdyk-Schauwecker, Shah, Murphy, Hastie, Mukherjee, Grdzelishvili: "Resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus: role of type I interferon signaling." in: Virology, Vol. 436, Issue 1, pp. 221-34, (2013) (PubMed).

Feijs, Kleine, Braczynski, Forst, Herzog, Verheugd, Linzen, Kremmer, Lüscher: "ARTD10 substrate identification on protein microarrays: regulation of GSK3β by mono-ADP-ribosylation." in: Cell communication and signaling : CCS, Vol. 11, Issue 1, pp. 5, (2013) (PubMed).

Hastie, Besmer, Shah, Murphy, Moerdyk-Schauwecker, Molestina, Roy, Curry, Mukherjee, Grdzelishvili: "Oncolytic vesicular stomatitis virus in an immunocompetent model of MUC1-positive or MUC1-null pancreatic ductal adenocarcinoma." in: Journal of virology, Vol. 87, Issue 18, pp. 10283-94, (2013) (PubMed).

Forst, Karlberg, Herzog, Thorsell, Gross, Feijs, Verheugd, Kursula, Nijmeijer, Kremmer, Kleine, Ladurner, Schüler, Lüscher: "Recognition of mono-ADP-ribosylated ARTD10 substrates by ARTD8 macrodomains." in: Structure (London, England : 1993), Vol. 21, Issue 3, pp. 462-75, (2013) (PubMed).

Goel, Sienkiewicz, Picciani, Wang, Lee, Bhattacharya: "Cochlin, intraocular pressure regulation and mechanosensing." in: PLoS ONE, Vol. 7, Issue 4, pp. e34309, (2012) (PubMed).

Goel, Sienkiewicz, Picciani, Lee, Bhattacharya: "Cochlin induced TREK-1 co-expression and annexin A2 secretion: role in trabecular meshwork cell elongation and motility." in: PLoS ONE, Vol. 6, Issue 8, pp. e23070, (2011) (PubMed).

Jesse, Koenig, Ellenrieder, Menke: "Lef-1 isoforms regulate different target genes and reduce cellular adhesion." in: International journal of cancer, Vol. 126, Issue 5, pp. 1109-20, (2010) (PubMed).

Shen, Da Silva, He, Cline: "Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 15, pp. 5032-43, (2009) (PubMed).

Forrest, Paden, Allen, Collins, Speck: "ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication." in: Journal of virology, Vol. 81, Issue 21, pp. 11957-71, (2007) (PubMed).

Yang, Camp, Gritsenko, Luo, Kelly, Clauss, Brinkley, Smith, Stenoien: "Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus." in: Journal of cell science, Vol. 120, Issue Pt 22, pp. 4060-70, (2007) (PubMed).

Koenig, Mueller, Hasel, Adler, Menke: "Collagen type I induces disruption of E-cadherin-mediated cell-cell contacts and promotes proliferation of pancreatic carcinoma cells." in: Cancer research, Vol. 66, Issue 9, pp. 4662-71, (2006) (PubMed).
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

Mouse anti-GFP antibody is functional by western blot, ELISA, Immunofluorescene Micorscopy and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
Synonyms: GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.