POLK 抗体 (AA 817-830)
Quick Overview for POLK 抗体 (AA 817-830) (ABIN129559)
抗原
See all POLK 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 817-830
-
Supplier Product No.
- 600-101-295
-
Supplier
- Rockland
-
原理
- POLK Antibody
-
交叉反应 （详细）
- This affinity purified antibody is directed against human POLK protein.
-
产品特性
- Synonyms: goat Anti-POLK antibody, goat Anti-DNA polymerase kappa antibody, DINB homolog 1 (E. coli) antibody, DINB protein antibody, DINB1 antibody, DINP antibody, DNA damage inducible protein b antibody, POLQ antibody
-
纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
-
过滤
- Sterile filtered
-
免疫原
-
Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Immunogen Type: Conjugated Peptide
-
亚型
- IgG
-
-
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-472) antibody
POLK 适用: 人 WB, ELISA, IF, IHC (p), RNAi 宿主: 小鼠 Monoclonal 6F2 unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-472) antibodyPOLK 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-473) antibodyPOLK 适用: 人 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 6F2 unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-472) antibodyPOLK 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 4F12 unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 227-337) antibodyPOLK 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-472) antibodyPOLK 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 1-472) antibodyPOLK 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 542-591) antibodyPOLK 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed) kappa (POLK) (AA 35-84) antibodyPOLK 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Application Note: This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLK by western blotting in the appropriate cell lysate or extract. Splice variants for this protein may show molecular weights of 89.0 and 76.4 kDa.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:2,000 - 1:10,000
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1.4 mg/mL
-
缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
有效期
- 12 months
-
-
- POLK (Polymerase (DNA Directed) kappa (POLK))
-
别名
- POLK
-
背景
- Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair. POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity. POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
-
基因ID
- 51426, 59798438
-
UniProt
- Q9UBT6
-
途径
- DNA Damage Repair
抗原
-
