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POLK 抗体 (AA 817-830)

Rockland 600-101-295 POLK 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
Rockland
产品编号 ABIN129559
Supplier Product No.: 600-101-295
发货至: 中国
  • 抗原 See all POLK 抗体
    POLK (Polymerase (DNA Directed) kappa (POLK))
    抗原表位
    • 15
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 817-830
    适用
    • 54
    • 15
    • 13
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    宿主
    • 47
    • 5
    • 2
    山羊
    克隆类型
    • 51
    • 3
    多克隆
    标记
    • 25
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLK antibody is un-conjugated
    应用范围
    • 28
    • 19
    • 14
    • 5
    • 4
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-101-295
    Supplier
    Rockland
    原理
    POLK Antibody
    交叉反应 (详细)
    This affinity purified antibody is directed against human POLK protein.
    产品特性
    Synonyms: goat Anti-POLK antibody, goat Anti-DNA polymerase kappa antibody, DINB homolog 1 (E. coli) antibody, DINB protein antibody, DINB1 antibody, DINP antibody, DNA damage inducible protein b antibody, POLQ antibody
    纯化方法
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    过滤
    Sterile filtered
    免疫原

    Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

    Immunogen Type: Conjugated Peptide

    亚型
    IgG
    Top Product
    Discover our top product POLK Primary Antibody
  • 应用备注

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLK by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 89.0 and 76.4 kDa.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.4 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    POLK (Polymerase (DNA Directed) kappa (POLK))
    别名
    POLK (POLK 产品)
    背景
    Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair.  POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity.  POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
    基因ID
    51426, 59798438
    UniProt
    Q9UBT6
    途径
    DNA Damage Repair
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