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UBA1 抗体

UBA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN129529
发货至: 中国
  • 抗原 See all UBA1 抗体
    UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
    适用
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    宿主
    • 69
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    克隆类型
    • 60
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    多克隆
    标记
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    This UBA1 antibody is un-conjugated
    应用范围
    • 35
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    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    产品特性
    Concentration Definition: by UV absorbance at 280 nm
    免疫原
    Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
    Immunogentype:Recombinant
    亚型
    IgG
    Top Product
    Discover our top product UBA1 Primary Antibody
  • 应用备注
    This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Restore with deionized water (or equivalent)
    浓度
    5.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
  • 抗原
    UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
    别名
    Ubiquitin Activating Enzyme E1 (UBA1 产品)
    背景
    Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation.  E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.  Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2.  Both variants encode the same protein.
    Synonyms: A1S9 antibody, A1S9 protein antibody, A1S9T and BN75 temperature sensitivity complementing antibody, A1S9T antibody, A1ST antibody, GXP 1 antibody
    基因ID
    7317, 23510338
    UniProt
    P22314
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