S100A1 抗体
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- 抗原 See all S100A1 抗体
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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适用
- 人, 小鼠, 大鼠, Cow
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This S100A1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Supplier Product No.
- 200-401-874
- Supplier
- Rockland
- 原理
- S100 Protein Antibody
- 交叉反应 （详细）
- This Protein A purified antibody is directed against bovine S100 protein.
- 产品特性
- Synonyms: rabbit anti-S100 Protein Antibody, S100 calcium-binding protein A1, S-100 protein subunit alpha, S-100 protein alpha chain
- 纯化方法
- The product was purified from monospecific antiserum by Protein A chromatography.
- 免疫原
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Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product S100A1 Primary Antibody
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anti-S100 Calcium Binding Protein A1 (S100A1) (N-Term) antibody
S100A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-75) antibodyS100A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal S100A1-1942 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-490R unconjugated Recombinant Antibody
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 2C8B8 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC, IF, StM 宿主: 小鼠 Monoclonal S1-61 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人, Cow WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 10-59) antibodyS100A1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 45-75) antibodyS100A1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: Cow WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:2,000
Application Note: This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 5.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
- 别名
- S100A1 (S100A1 产品)
- 背景
- Background: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21 kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - bb homodimer (S-100b) and ab heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
- 基因ID
- 528735
- NCBI登录号
- NP_001092512
- UniProt
- P02639
- 途径
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
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