NEDD9 抗体 (AA 82-398)
(1 reference)
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- 抗原 See all NEDD9 抗体
- NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
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抗原表位
- AA 82-398
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NEDD9 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- Supplier Product No.
- 200-301-904
- Supplier
- Rockland
- 原理
- HEF1 Antibody
- 交叉反应 （详细）
- This Protein A purified antibody is directed against human HEF1 protein.
- 产品特性
- Synonyms: mouse anti-hEF1 antibody, mouse anti-NEDD-9 antibody, mouse anti-CASL antibody, Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody
- 纯化方法
- The product was purified from tissue culture supernatant by chromatography.
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein (hHEF1, 843 aa, predicted MW 92.8 kDa).
Immunogen Type: Conjugated Peptide
- 克隆位点
- 2G9
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NEDD9 Primary Antibody
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 60-174) antibody
NEDD9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 101-200) antibodyNEDD9 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B4 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 82-398) antibodyNEDD9 适用: 人, 大鼠, 小鼠 WB, IF, IP, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 2G9 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (Internal Region) antibodyNEDD9 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 1-834) antibodyNEDD9 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 1-834) antibodyNEDD9 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) antibodyNEDD9 适用: 人, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 335-363) antibodyNEDD9 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 82-398) antibodyNEDD9 适用: 人 WB, ELISA, IF, IP, FM 宿主: 小鼠 Monoclonal 14A11 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9) (AA 68-151) antibodyNEDD9 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Application Note: This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract. This antibody does not recognize p130Cas. Sin1 has not been tested. IF was performed using 4 % PFA fixed cells. This monoclonal antibody mostly detects HEF1 localized at the focal adhesion sites.
Western Blot Dilution: 1:5,000
Immunoprecipitation Dilution: 1:1,000
ELISA Dilution: 1:5,000 - 1:20,000
IF Microscopy Dilution: 1:500
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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: "The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome." in: Nature cell biology, Vol. 7, Issue 10, pp. 937-46, (2005) (PubMed).
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: "The focal adhesion scaffolding protein HEF1 regulates activation of the Aurora-A and Nek2 kinases at the centrosome." in: Nature cell biology, Vol. 7, Issue 10, pp. 937-46, (2005) (PubMed).
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- 抗原
- NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))
- 别名
- NEDD9 (NEDD9 产品)
- 背景
- Background: HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene. HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form. HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas. HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1. This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus. HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue. HEF1 is also detected in T-cells, B-cells and diverse cell lines. HEF1 is activated upon induction of cell growth. Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55. Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle. Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected. Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.
- 基因ID
- 4739, 5453680
- UniProt
- Q14511
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